Structural dissection of the reaction mechanism of cellobiose phosphorylase.

The Biochemical Journal
Masafumi HidakaShinya Fushinobu

Abstract

Cellobiose phosphorylase, a member of the glycoside hydrolase family 94, catalyses the reversible phosphorolysis of cellobiose into alpha-D-glucose 1-phosphate and D-glucose with inversion of the anomeric configuration. The substrate specificity and reaction mechanism of cellobiose phosphorylase from Cellvibrio gilvus have been investigated in detail. We have determined the crystal structure of the glucose-sulphate and glucose-phosphate complexes of this enzyme at a maximal resolution of 2.0 A (1 A=0.1 nm). The phosphate ion is strongly held through several hydrogen bonds, and the configuration appears to be suitable for direct nucleophilic attack to an anomeric centre. Structural features around the sugar-donor and sugar-acceptor sites were consistent with the results of extensive kinetic studies. When we compared this structure with that of homologous chitobiose phosphorylase, we identified key residues for substrate discrimination between glucose and N-acetylglucosamine in both the sugar-donor and sugar-acceptor sites. We found that the active site pocket of cellobiose phosphorylase was covered by an additional loop, indicating that some conformational change is required upon substrate binding. Information on the three-dimen...Continue Reading

References

Jul 1, 1992·Journal of Biochemistry·M KitaokaH Taniguchi
Aug 1, 1993·Archives of Biochemistry and Biophysics·M KitaokaH Taniguchi
May 1, 1997·Nature Structural Biology·M O'ReillyL N Johnson
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
May 20, 1999·Nature Structural Biology·A PerrakisV S Lamzin
Apr 26, 2001·Analytical Biochemistry·M KitaokaK Hayashi
Sep 10, 2002·Journal of Molecular Biology·S GeremiaL N Johnson
Feb 25, 2003·Bioscience, Biotechnology, and Biochemistry·Eranna RajashekharaKiyoshi Hayashi
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Sep 25, 2004·Acta Crystallographica. Section D, Biological Crystallography·Masafumi HidakaShinya Fushinobu
Feb 19, 2005·The Journal of Biological Chemistry·Shinya FushinobuMotomitsu Kitaoka
Oct 18, 2005·Carbohydrate Research·Mark R StamBernard Henrissat
Jan 1, 1997·Methods in Enzymology·E A Merritt, D J Bacon
Jan 1, 1992·Bioscience, Biotechnology, and Biochemistry·M KitaokaH Taniguchi
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Aug 3, 2011·Applied Microbiology and Biotechnology·Takanori NihiraMotomitsu Kitaoka
Sep 6, 2013·Journal of Industrial Microbiology & Biotechnology·Nguyen Huy Thuan, Jae Kyung Sohng
Jan 7, 2009·The Journal of Biological Chemistry·Masafumi HidakaShinya Fushinobu
Jun 3, 2009·Protein Engineering, Design & Selection : PEDS·Manu R M De GroeveWim Soetaert
Mar 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Annelies Van HoorebekeSavvas N Savvides
Nov 22, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Christopher M BianchettiGeorge N Phillips
Sep 6, 2011·Applied and Environmental Microbiology·Michael IakiviakIsaac K O Cann
Dec 15, 2010·Bioscience, Biotechnology, and Biochemistry·Shinya Fushinobu
Feb 9, 2012·Bioscience, Biotechnology, and Biochemistry·Motomitsu KitaokaKiyoshi Hayashi
Mar 22, 2014·PloS One·Masahiro NakajimaMotomitsu Kitaoka
Jun 26, 2007·Bioscience, Biotechnology, and Biochemistry·Mamoru Nishimoto, Motomitsu Kitaoka
Aug 2, 2011·Journal of Biotechnology·Manu R M de GroeveWim Soetaert
Jun 12, 2010·Computational Biology and Chemistry·Nalin C W GoonesekereKevin O'Connor
Jun 17, 2010·Biotechnology and Bioengineering·Thu V Vuong, David B Wilson
Mar 2, 2010·Analytical Biochemistry·M R M De GroeveW Soetaert
Aug 22, 2015·Applied Microbiology and Biotechnology·Motomitsu Kitaoka
Feb 14, 2013·Current Opinion in Chemical Biology·Hiroyuki NakaiKen'ichi Ohtsubo
Sep 25, 2010·Journal of Molecular Biology·Malene Bech Vester-ChristensenAnette Henriksen
Sep 28, 2016·ACS Synthetic Biology·Kulika ChomvongJamie H D Cate
May 31, 2015·Applied and Environmental Microbiology·Patricia A HingstonLisbeth Truelstrup Hansen
Aug 15, 2013·The Journal of Biological Chemistry·Takanori NihiraHiroyuki Nakai
Jan 11, 2018·The Journal of Biological Chemistry·Sakonwan KuhaudomlarpRobert A Field
Mar 6, 2021·Synthetic and Systems Biotechnology·Shangshang Sun, Chun You
May 11, 2021·Applied Microbiology and Biotechnology·Zorica UbiparipTom Desmet

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