Structural diversity and specific distribution of O-glycans in normal human mucins along the intestinal tract

The Biochemical Journal
Catherine RobbeJean-Claude Michalski

Abstract

Purified human mucins from different parts of the intestinal tract (ileum, cecum, transverse and sigmoid colon and rectum) were isolated from two individuals with blood group ALe(b) (A-Lewis(b)). After alkaline borohydride treatment the released oligosaccharides were structurally characterized by nano-ESI Q-TOF MS/MS (electrospray ionization quadrupole time-of-flight tandem MS) without prior fractionation or derivatization. More than 100 different oligosaccharides, with up to ten monosaccharide residues, were identified using this technique. Oligosaccharides based on core 3 structures, GlcNAc(beta1-3)GalNAc (where GlcNAc is N-acetyl-D-glucosamine and GalNAc is N-acetylgalactosamine), were widely distributed in human intestinal mucins. Core 5 structures, GalNAc(alpha1-3)GalNAc, were also recovered in all fractions. Moreover, a comparison of the oligosaccharide repertoire, with respect to size, diversity and expression of glycans and terminal epitopes, showed a high level of mucin-specific glycosylation: highly fucosylated glycans, found specifically in the small intestine, were mainly based on core 4 structures, GlcNAc-(beta1-3)[GlcNAc(beta1-6)]GalNAc, whereas the sulpho-Le(X) determinant carrying core 2 glycans, Gal(beta1-3)[Gl...Continue Reading

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Citations

Nov 11, 2008·Cancer Chemotherapy and Pharmacology·Andrea M StringerDorothy M K Keefe
Sep 25, 2007·Glycoconjugate Journal·Shin-Yi YuAlbert M Wu
Sep 23, 2008·Glycoconjugate Journal·Catherine Robbe-MasselotCalliope Capon
Nov 20, 2012·Nature·Alline R PachecoVanessa Sperandio
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Matti KankainenWillem M de Vos
Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Martin StahlAlain Stintzi
May 31, 2013·Glycobiology·Angela MarcobalJustin L Sonnenburg
Jun 12, 2013·Glycobiology·Kirk S B Bergstrom, Lijun Xia
Mar 16, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Patrick GunningNathalie Juge
Jan 15, 2013·Applied and Environmental Microbiology·Seema DasPrasad Gyaneshwar
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Feb 21, 2014·Foodborne Pathogens and Disease·Sahyun HongYeon-Ho Kang
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Nov 18, 2011·Trends in Microbiology·Nathalie Juge

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