Structural diversity in bacterial ribosomes: mycobacterial 70S ribosome structure reveals novel features

PloS One
Manidip Shasmal, Jayati Sengupta

Abstract

Here we present analysis of a 3D cryo-EM map of the 70S ribosome from Mycobacterium smegmatis, a saprophytic cousin of the etiological agent of tuberculosis in humans, Mycobacterium tuberculosis. In comparison with the 3D structures of other prokaryotic ribosomes, the density map of the M. smegmatis 70S ribosome reveals unique structural features and their relative orientations in the ribosome. Dramatic changes in the periphery due to additional rRNA segments and extra domains of some of the peripheral ribosomal proteins like S3, S5, S16, L17, L25, are evident. One of the most notable features appears in the large subunit near L1 stalk as a long helical structure next to helix 54 of the 23S rRNA. The sharp upper end of this structure is located in the vicinity of the mRNA exit channel. Although the M. smegmatis 70S ribosome possesses conserved core structure of bacterial ribosome, the new structural features, unveiled in this study, demonstrates diversity in the 3D architecture of bacterial ribosomes. We postulate that the prominent helical structure related to the 23S rRNA actively participates in the mechanisms of translation in mycobacteria.

References

Jan 1, 1979·International Archives of Allergy and Applied Immunology·R BakerO Ouchterlony
Jan 1, 1974·Molecular & General Genetics : MGG·C C Sherton, I G Wool
Jul 1, 1973·Cellular Immunology·N HannaS LESKOWITZ
Feb 1, 1994·Antimicrobial Agents and Chemotherapy·N Honoré, S T Cole
Jul 1, 1993·Nucleic Acids Research·J M NeefsR De Wachter
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·J P McCutcheonJ Frank
Mar 18, 2000·Cell·I S GabashviliP A Penczek
Apr 3, 2001·Science·Marat YusupovHarry F Noller
Mar 23, 2002·Cell·V Ramakrishnan
Jul 16, 2003·Cell·Mikel ValleJoachim Frank
Oct 21, 2003·Nature Structural Biology·Mikel ValleJoachim Frank
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Jul 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Haixiao GaoJoachim Frank
Nov 2, 2005·Nature Reviews. Microbiology·Jacob Poehlsgaard, Stephen Douthwaite
Mar 24, 2006·Molecular Microbiology·Tanel Tenson, Alexander S Mankin
Jun 9, 2006·Trends in Microbiology·Liem Nguyen, Charles J Thompson
Sep 9, 2006·Science·Maria SelmerV Ramakrishnan
Oct 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Veysel BerkJamie H Doudna Cate
Oct 20, 2006·Nature·Gulnara YusupovaMarat Yusupov
Nov 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Joachim FrankDerek J Taylor
Jun 10, 2008·Molecular Cell·Peter V CornishTaekjip Ha
Oct 14, 2008·Current Opinion in Chemical Biology·Andrei KorostelevHarry F Noller
Oct 28, 2008·Biological Chemistry·Stefano MarziThomas Geissmann
Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth VillaJoachim Frank
Oct 20, 2009·Nature·T Martin Schmeing, V Ramakrishnan

Citations

Jun 21, 2014·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Jian WeiNa Guo
Mar 19, 2014·Amino Acids·Michael S ParkerSteven L Parker
Mar 15, 2013·FEBS Letters·Aleksandra Filipovska, Oliver Rackham
Nov 23, 2017·Frontiers in Microbiology·Karolina DaduraJarosław Dziadek
Jun 6, 2012·Nature Structural & Molecular Biology·Sergey MelnikovMarat Yusupov
Jun 28, 2018·Nucleic Acids Research·Elizabeth B SawyerTeresa Cortes
Dec 21, 2019·Progress in Biophysics and Molecular Biology·Ambuj Kumar Kushwaha, Shashi Bhushan

Datasets Mentioned

BETA
EMD-5307

Related Concepts

Genus Mycobacterium
Mycobacterium tuberculosis H37Rv
Protein Conformation
Ribosomal Proteins
Ribosomes
15S RNA
Protein Biosynthesis
Mycobacterium smegmatis
Cryoelectron Microscopy
Ribosome Subunits, Small, Bacterial

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