Structural diversity of sphingomyelin microdomains

Ultramicroscopy
Marie-Cécile GiocondiChristian Le Grimellec

Abstract

In cells plasma membrane, sphingomyelin (SM) plays a key role in the formation of a category of lipid microdomains enriched in cholesterol (Chl) often referred to as rafts. Atomic force microscopy (AFM) was used to analyze the mesoscopic topography of enriched SM microdomains in supported bilayers made of SM/dioleoylphosphatidylcholine (SM/DOPC) and SM/palmitoyl-oleoyl-phosphatidylcholine (SM/POPC) equimolar mixtures, in buffer, at room temperature. Gel-fluid phase separation occurs in both SM/DOPC and SM/POPC bilayers. The gel phase SM-enriched microdomains adopt a variety of size, shape and mesoscopic structure, from homogeneous flat domains of a few hundreds of nanometer in diameter to domains of several micrometers made of closely packed globular structures. Gel-gel phase separation in SM domains is also observed which gives rise to different structures for the diunsaturated and the mixed-saturated PC species. These differences could also extend to the interactions with Chl. This suggests that studies on rafts formation commonly performed using SM/DOPC mixture as a model should also include the physiologically more relevant POPC species.

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Citations

Dec 7, 2007·Pflügers Archiv : European journal of physiology·Marie-Cécile GiocondiChristian Le Grimellec
Oct 9, 2008·Journal of the American Chemical Society·Tim BartelsMichael F Brown
May 1, 2007·The Journal of Membrane Biology·Robert J O'ConnellSteven N Treistman
Jan 28, 2016·Colloids and Surfaces. B, Biointerfaces·Andreia CucoAmélia Gonçalves da Silva
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Apr 29, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Soichiro MatsunagaMaki Kawai
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Jun 15, 2007·Biochimica Et Biophysica Acta·Karim El Kirat, Sandrine Morandat
Oct 3, 2009·Biochimica Et Biophysica Acta·Marie-Cécile GiocondiChristian Le Grimellec
Aug 12, 2009·Biochimica Et Biophysica Acta·Karim El KiratYves F Dufrêne
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Aug 19, 2007·Journal of Molecular Recognition : JMR·Marie-Cécile GiocondiChristian Le Grimellec
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Nov 2, 2019·Biochimica Et Biophysica Acta. Biomembranes·Arturo Galván-HernándezIván Ortega-Blake
Jan 25, 2020·Biochimie·Sathishkumar MunusamyCarlos Munoz-Garay
Jul 31, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Marie-Cécile GiocondiChristian Le Grimellec
May 25, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Olaf UhlKeith M Godfrey
Jan 21, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Drew MarquardtGeorg Pabst
Feb 14, 2006·The Journal of Physical Chemistry. B·Victor V VolkovRoberto Righini
Jan 30, 2004·Biophysical Journal·Marie-Cécile GiocondiChristian Le Grimellec
Sep 2, 2020·Biochimica Et Biophysica Acta. Biomembranes·Romina F VázquezSabina M Maté

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