Structural domains of Porphyromonas gingivalis recombinant fimbrillin that mediate binding to salivary proline-rich protein and statherin.

Infection and Immunity
A AmanoR J Genco

Abstract

Fimbriae (the oligomeric form of fimbrillin) are considered important in the adherence and colonization of Porphyromonas gingivalis in the oral cavity. In the present study, we have identified the structural domains of P. gingivalis fimbrillin that mediate the binding to salivary proline-rich protein 1 (PRP1) and statherin. A series of synthetic fimbrillin peptides were used to localize the active fimbrillin domains involved in the binding to PRP1 and statherin. The binding of 125I-labeled 41-r-Fim (whole-length recombinant fimbrillin, amino acid [aa] residues 1 to 337) to PRP1-coated hydroxyapatite beads (HAP) was strongly inhibited by the fimbrillin C-terminal peptides corresponding to aa residues 266 to 286 and 318 to 337 (peptides 266-286, and 318-337, respectively), while the binding to statherin was inhibited by C-terminal peptides 266-286, 293-306 and 307-326. Peptide 126-146 also showed a weak inhibitory effect, about half that of other active peptides, on the binding to both PRP1 and statherin. P. gingivalis whole-cell binding to PRP1- or statherin-coated HAP was inhibited by more than 80% by the same active peptides. To confirm that the C-terminal portion of fimbrillin includes domains responsible for the binding, two...Continue Reading

References

Mar 15, 1991·Biochemical and Biophysical Research Communications·H T SojarR J Genco
Jan 1, 1990·Archives of Oral Biology·R J GibbonsG Davis
Mar 1, 1988·Microbiological Reviews·D Mayrand, S C Holt
Jun 1, 1995·Oral Microbiology and Immunology·E E BrantE De Nardin
Aug 1, 1993·Oral Microbiology and Immunology·Y NaitoI Takazoe
May 1, 1994·Infection and Immunity·N HamadaT Umemoto
Aug 1, 1994·Infection and Immunity·A AmanoR J Genco

❮ Previous
Next ❯

Citations

Feb 15, 2002·Advances in Dental Research·J D Rudney
Sep 1, 2005·The Journal of Biological Chemistry·George HajishengallisEvlambia Harokopakis
Aug 26, 2006·Periodontology 2000·Mary E Davey, John W Costerton
Aug 18, 2010·Periodontology 2000·Masae KuboniwaAtsuo Amano
Jan 12, 2001·Oral Microbiology and Immunology·R J Lamont, H F Jenkinson
Nov 28, 2000·Journal of Bacteriology·H XieR J Lamont
May 25, 2002·Cellular Microbiology·Ozlem YilmazRichard J Lamont
Nov 3, 2009·Periodontology 2000·Sven-Ulrik Gorr
Sep 30, 2010·Journal of Biomedical Materials Research. Part a·Yuta TanakaTakao Hanawa
Dec 17, 2009·Clinical Implant Dentistry and Related Research·Masanori AokiKazuyuki Ishihara
Mar 27, 1999·Oral Microbiology and Immunology·S HamadaI Morisaki
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Evlambia HarokopakisGeorge Hajishengallis
Jun 10, 2009·Molecular Biotechnology·Eun-Ah ShinJin-Yong Lee
Oct 9, 2007·The Biochemical Journal·Periathamby Antony RajAndrew R Dentino
May 18, 2010·Journal of Peptide Science : an Official Publication of the European Peptide Society·Barbara ManconiMaria Teresa Sanna
Sep 13, 2008·Critical Reviews in Food Science and Nutrition·Martha R Bajec, Gary J Pickering
Dec 19, 2009·Periodontology 2000·Atsuo Amano
Sep 3, 2013·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Keiji Nagano
Mar 5, 2002·Clinical and Diagnostic Laboratory Immunology·George HajishengallisRobert J Genco
Jan 10, 2006·Periodontology 2000·Zhimin Feng, Aaron Weinberg
Nov 18, 2000·Infection and Immunity·H XieR J Lamont
Nov 13, 2004·The Bulletin of Tokyo Dental College·Kiyotoshi TakanashiKazuyuki Ishihara
Jun 24, 2004·Oral Microbiology and Immunology·C G MissailidisM P A Mayer
Dec 22, 2020·Current Oral Health Reports·George Hajishengallis, Patricia I Diaz
Oct 24, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Ye Jin, Hak-Kong Yip
Oct 8, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Patrick S StaytonMichele Gilbert
Aug 2, 2017·Periodontology 2000·Jeffrey L EbersoleOctavio A Gonzalez
Sep 23, 1997·Biochemical and Biophysical Research Communications·A SharmaR J Genco
Aug 26, 1998·Biochemical and Biophysical Research Communications·M KuboniwaS Shizukuishi
May 1, 1999·Biochemical and Biophysical Research Communications·A SharmaR J Genco
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·R J Lamont, H F Jenkinson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.