Structural dynamics of the magnesium-bound conformation of CorA in a lipid bilayer.

Structure
Olivier DalmasEduardo Perozo

Abstract

The transmembrane conformation of Thermotoga maritima CorA, a magnesium transport system, has been studied in its Mg(2+)-bound form by site-directed spin labeling and electron paramagnetic resonance spectroscopy. Probe mobility together with accessibility data were used to evaluate the overall dynamics and relative arrangement of individual transmembrane segments TM1 and TM2. TM1 extends toward the cytoplasmic side creating a water-filled cavity, while TM2 is located in the periphery of the oligomer, contacting the lipid bilayer. A structural model for the conserved extracellular loop was generated based on EPR data and MD simulations, in which residue E316 is located toward the five-fold symmetry axis in position to electrostatically influence divalent ion translocation. Electrostatic analysis of our model suggest that, in agreement with the crystal structure, Mg(2+) -bound CorA is in a closed conformation. The present results suggest that long-range structural rearrangements are necessary to allow Mg(2+) translocation.

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Citations

Apr 4, 2014·Nature Communications·Olivier DalmasEduardo Perozo
Feb 26, 2014·The Journal of General Physiology·Yuichiro FujiwaraYasushi Okamura
Feb 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Olivier DalmasEduardo Perozo
Jun 23, 2012·The Journal of Biological Chemistry·Isolde PalomboMikaela Rapp
Sep 12, 2012·Journal of Statistical Physics·David M RogersSusan B Rempe
Jul 20, 2010·Structure·Yeon-Kyun Shin
Aug 21, 2013·Biochimica Et Biophysica Acta·Jian PayandehEmil F Pai
Oct 27, 2016·The Journal of Chemical Physics·Sunan KitjaruwankulR B Pandey
Oct 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Albert GuskovSaid Eshaghi
Jan 15, 2014·Journal of Bacteriology·Catherine A WakemanWade C Winkler
Nov 28, 2019·ELife·Martina RanglSimon Scheuring
Nov 6, 2018·The Journal of Physical Chemistry. B·Fabián G Cantú ReinhardSam P de Visser

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