Structural dynamics studies on the binding of aflatoxin B1 to chicken egg albumin using spectroscopic techniques and molecular docking.

Journal of Biomolecular Structure & Dynamics
Mohd Aamir Qureshi, Saleem Javed

Abstract

Aflatoxin B1, a mycotoxin produced by large number of Aspergillus species including Aspergillus flavus and Aspergillus parasiticus, has been described as the most potent carcinogenic mycotoxin. In this study, we have used a multiple spectroscopic and molecular docking approach to investigate the interaction of aflatoxin B1 (AFB1) with chicken egg albumin (CEA). Fluorescence spectroscopy, UV-Vis spectroscopy, and three-dimensional fluorescence spectroscopic techniques were employed to gain insight into the conformational changes in CEA in the presence of AFB1. Fluorescence spectroscopy revealed ligand-induced quenching in the fluorescence emission spectra of CEA upon binding with AFB1. Hyperchromic effect was observed in case of the ground state complex formation between CEA and AFB1 by UV-Vis spectroscopy. To gain further comprehension into the site of binding of AFB1 to CEA, competitive site marker displacement assay was performed using warfarin site marker. The magnitude of ΔG value calculated from fluorescence-based method was negative which confirmed spontaneous process. The results obtained suggest that the binding is enthalpy driven and van der Waals force and hydrogen bonds are stabilizing the AFB1-CEA complex. Three-dim...Continue Reading

References

Jun 29, 1978·Nature·L McReynoldsG G Brownlee
Oct 5, 1991·Journal of Molecular Biology·P E SteinR W Carrell
Oct 1, 1973·Photochemistry and Photobiology·E A BursteinM N Ivkova
Feb 7, 1998·Journal of Veterinary Pharmacology and Therapeutics·H RajaianC J Bowmer
Jun 26, 2003·Critical Reviews in Food Science and Nutrition·H N Mishra, Chitrangada Das
Oct 29, 1963·Biochimica Et Biophysica Acta·L W CUNNINGHAMJ D FORD
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Nov 10, 2004·Journal of Pharmaceutical and Biomedical Analysis·Yan-Jun HuSong-Sheng Qu
Dec 23, 2004·Systematic and Applied Microbiology·Jens C Frisvad, Robert A Samson
Jan 18, 2006·Journal of Pharmaceutical and Biomedical Analysis·P B KandagalO B Ijare
Apr 15, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Peter F SchollChristopher P Wild
Apr 29, 2009·Journal of Computational Chemistry·Garrett M MorrisArthur J Olson
Feb 23, 2010·Journal of Biochemical and Molecular Toxicology·Emna El Golli-BennourHassen Bacha
Apr 2, 2010·Archives of Microbiology·M Z AbdinSaleem Javed
Aug 27, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Laura-Ana CorcueraAdela López de Cerain
Aug 15, 2012·Journal of Cheminformatics·Marcus D HanwellGeoffrey R Hutchison
Nov 16, 2012·Current Protocols in Protein Science·David T Clarke
Dec 26, 2012·Molecular Biology Reports·Rui-Qiang WangRui-Yong Wang
Nov 16, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Wenli XieJian Shen
Jul 6, 2014·Food Chemistry·Jana OgnjenovićTanja Cirkovic Velickovic
Nov 11, 2014·Molecular BioSystems·Sumit Kumar ChaturvediRizwan Hasan Khan
Apr 22, 2017·International Journal of Biological Macromolecules·Maria Dayanne de A DantasJosué Carinhanha Caldas Santos
Jul 12, 2017·International Journal of Biological Macromolecules·Mohammad Khursheed SiddiqiRizwan Hasan Khan
Aug 19, 2017·International Journal of Biological Macromolecules·Anali MansouriMojtaba Falahati
Feb 27, 2018·International Journal of Biological Macromolecules·Azaj AhmedBilqees Bano
Mar 8, 2018·Journal of Biomolecular Structure & Dynamics·Shumaila AfrinMohammad Tabish
Jun 3, 2018·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Gufran Ahmed SiddiquiAabgeena Naeem
Aug 28, 2018·International Journal of Biological Macromolecules·Mohammad Khalid ZiaFahim Halim Khan

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
circular dichroism
Fluorescence
FRET
fluorescence spectroscopy
fluorescence spectroscopic studies

Software Mentioned

Autodock
PhotochemCAD
Ligplot
Avogadro
USIF Chimera

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.