Structural effects of the antimicrobial peptide maculatin 1.1 on supported lipid bilayers.

European Biophysics Journal : EBJ
David I FernandezFrances Separovic

Abstract

The interactions of the antimicrobial peptide maculatin 1.1 (GLFGVLAKVAAHVVPAIAEHF-NH(2)) with model phospholipid membranes were studied by use of dual polarisation interferometry and neutron reflectometry and dimyristoylphosphatidylcholine (DMPC) and mixed DMPC-dimyristoylphosphatidylglycerol (DMPG)-supported lipid bilayers chosen to mimic eukaryotic and prokaryotic membranes, respectively. In DMPC bilayers concentration-dependent binding and increasing perturbation of bilayer order by maculatin were observed. By contrast, in mixed DMPC-DMPG bilayers, maculatin interacted more strongly and in a concentration-dependent manner with retention of bilayer lipid order and structure, consistent with pore formation. These results emphasise the importance of membrane charge in mediating antimicrobial peptide activity and emphasise the importance of using complementary methods of analysis in probing the mode of action of antimicrobial peptides.

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Citations

Oct 25, 2012·Physical Chemistry Chemical Physics : PCCP·David I FernandezFrances Separovic
Mar 7, 2014·Biochimica Et Biophysica Acta·Tzong-Hsien LeeMarie-Isabel Aguilar
Nov 13, 2013·Biochimica Et Biophysica Acta·Parvesh WadhwaniAnne S Ulrich
Jun 17, 2015·Biochimica Et Biophysica Acta·Marc-Antoine SaniFrances Separovic
Jun 12, 2013·Biochimica Et Biophysica Acta·Vinod BalharaChristine Dewolf
Nov 5, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Florian GraefClaus-Michael Lehr
May 10, 2017·European Biophysics Journal : EBJ·Marc-Antoine SaniFrances Separovic
Aug 1, 2020·Frontiers in Chemistry·Anton P Le BrunFrances Separovic
Nov 29, 2013·Journal of Labelled Compounds & Radiopharmaceuticals·Tamim A DarwishMichael James
Nov 2, 2019·Biochimica Et Biophysica Acta. Biomembranes·Arturo Galván-HernándezIván Ortega-Blake
Jan 26, 2020·Biochimica Et Biophysica Acta. Biomembranes·Marc-Antoine SaniFrances Separovic
Jun 17, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Tania Kjellerup LindHanna Pauliina Wacklin
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Mar 30, 2013·Biomacromolecules·Shalini SinghMartin Malmsten
Jan 10, 2019·Chemical Reviews·Shantanu GuhaWilliam C Wimley
Sep 25, 2021·Faraday Discussions·Tzong-Hsien LeeMarie-Isabel Aguilar

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