PMID: 44463Jan 1, 1979

Structural elements in adenovirus cores. Evidence for a "core shell" and linear structures in "relaxed" cores

Archives of Virology
M V Nermut

Abstract

"Freeze-fracture negative staining" of adenovirus type 5 revealed the virus cores as internal bodies with a fine granular surface which at high magnification shows reticular and ring-like patterns. This indicates that the virus protein V forms a thin surface layer for which a name "core shell" is proposed. Virus cores prepared by heating virus particles in sodium deoxycholate (DOC) relaxed into curved filaments or several rods by means of EGTA and high salt, respectively. High pH treatment had similar effects as high salt. Rod-like elements were also observed in ultrathin sections of the "DOC-cores". Fresh cores exhibited circular dichroism (C.D.) with similar features as described for nucleosomes (COWMAN and FASMAN, ref. 5). This indicates that the DNA has an orderly arrangement in the cores. EGTA had no effect on C.D. spectra but high salt treatment abolished the positive peak in 10 minutes. It is concluded that the adenovirus nucleocapsid is a linear structure, presumably a 120-150 Angstrom thick filament folded 5 to 6 times into "rods" as previously observed by freeze-fracturing.

References

Dec 7, 2010·Applied and Environmental Microbiology·Anne C Eischeid, Karl G Linden

Citations

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M K Cowman, G D Fasman
Nov 1, 1977·Nucleic Acids Research·H J LiC Chang
Jul 1, 1975·The Journal of General Virology·M V NermutW C Russell
Feb 1, 1977·Nucleic Acids Research·J A HarpstW C Russell
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J CordenG D Pearson
Aug 1, 1975·Journal of Virology·D T BrownW Doerfler
Jan 1, 1969·Advances in Immunology·N M Green
Apr 1, 1971·Journal of Ultrastructure Research·J DubochetE Kellenberger
Apr 1, 1971·The Journal of General Virology·W C RussellJ J Skehel
Nov 14, 1968·Journal of Molecular Biology·W G LaverR C Valentine
Oct 1, 1967·The Journal of General Virology·W C RussellH G Pereira

Related Concepts

Nucleosomes
Virion
Viral Proteins
Deoxycholic Acid, Monoammonium Salt
DNA, Viral
Hot Temperature
Sputolysin
Egtazic Acid
Nucleocapsid
Viral Nucleocapsid Location

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