Abstract
Escherichia coli YicI, a member of glycoside hydrolase family (GH) 31, is an alpha-xylosidase, although its amino-acid sequence displays approximately 30% identity with alpha-glucosidases. By comparing the amino-acid sequence of GH 31 enzymes and through structural comparison of the (beta/alpha)(8) barrels of GH 27 and GH 31 enzymes, the amino acids Phe277, Cys307, Phe308, Trp345, Lys414, and beta-->alpha loop 1 of (beta/alpha)(8) barrel of YicI have been identified as elements that might be important for YicI substrate specificity. In attempt to convert YicI into an alpha-glucosidase these elements have been targeted by site-directed mutagenesis. Two mutated YicI, short loop1-enzyme and C307I/F308D, showed higher alpha-glucosidase activity than wild-type YicI. C307I/F308D, which lost alpha-xylosidase activity, was converted into alpha-glucosidase.
References
Dec 1, 1991·The Biochemical Journal·B Henrissat
Aug 1, 1993·The Biochemical Journal·B Henrissat, A Bairoch
Sep 15, 1995·Structure·G Davies, B Henrissat
Jun 1, 1996·The Biochemical Journal·B Henrissat, A Bairoch
Aug 14, 1999·Biochimica Et Biophysica Acta·S YuJ Marcussen
May 10, 2000·The Journal of Biological Chemistry·M MoracciM Rossi
May 12, 2000·Plant Physiology·T P FrandsenB Svensson
Sep 12, 2000·Bioinformatics·L Holm, J Park
Apr 12, 2001·European Journal of Biochemistry·M OkuyamaS Chiba
Jul 3, 2002·Bioscience, Biotechnology, and Biochemistry·Hajime AgaYoshio Tsujisaka
Jul 19, 2002·FEBS Letters·Daniel J Rigden
Mar 27, 2003·The Journal of Biological Chemistry·Zui FujimotoHiroshi Mizuno
Nov 5, 2003·Biochemistry·Seung Seo LeeStephen G Withers
Aug 6, 2004·Protein Expression and Purification·Masayuki OkuyamaAtsuo Kimura
Oct 27, 2004·The Journal of Biological Chemistry·Andrew L LoveringNatalie C J Strynadka
Jun 2, 2005·Angewandte Chemie·Manfred T ReetzAndreas Vogel
Citations
Mar 31, 2010·Applied Microbiology and Biotechnology·Douglas B Jordan, Kurt Wagschal
Feb 25, 2014·Bioresource Technology·Micky VincentJ Hans van Leeuwen
Sep 27, 2013·PloS One·Marcelo M ZerilloNed Tisserat
Dec 8, 2011·Bioscience, Biotechnology, and Biochemistry·Masayuki Okuyama
Mar 14, 2007·FEBS Letters·Stefan JanecekE Ann MacGregor
Apr 15, 2016·Journal of Bioscience and Bioengineering·Tomohiko MatsuzawaKatsuro Yaoi
Mar 24, 2011·The Biochemical Journal·Johan LarsbrinkHarry Brumer
Aug 2, 2013·The Journal of Biological Chemistry·Henriëtte J RozeboomBauke W Dijkstra
Sep 4, 2015·The Journal of Biological Chemistry·Yuka OkazawaTakashi Tonozuka
Mar 13, 2020·ACS Sustainable Chemistry & Engineering·Hongnan CaoGeorge N Phillips
Jan 1, 2022·Applied Microbiology and Biotechnology·Tomohiko MatsuzawaKatsuro Yaoi