PMID: 8987862Nov 1, 1996Paper

Structural elucidation of N-liked sugar chains of storage glycoproteins in mature pea (Pisum sativum) seeds by ion-spray tandem mass spectrometry (IS-MS/MS)

Bioscience, Biotechnology, and Biochemistry
Y KimuraS Takagi

Abstract

The structures of N-linked sugar chains of the storage glycoproteins in mature pea seeds have been estimated. Nine pyridylaminated (PA-) N-linked sugar chains were derived and purified from the hydrazinolysate of the storage glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were first identified by two-dimensional PA-sugar chain mapping, considering the data of sugar composition analysis or sequential exoglycosidase digestions. The deduced structures were further analyzed by IS-MS/MS analysis. Every relevant fragment ion derived from all PA-sugar chains could be assigned on the basis of deduced structures. The estimated nine structures fell into two categories; the first was a typical oligomannose type (Man8-3GlcNAc2; 77.7%) which can be hydrolyzed by endo-beta-N-acetylglucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228-232 (1996)], the second was a xylose-containing type (Man4-3Xyl1GlcNAc2, Man3Fuc1Xyl1GlcNAc2; 22.3%). Among these structures, Man8GlcNAc2 (19.7%), Man6GlcNAc2 (24.7%), and Man3Fuc1Xyl1GlcNAc2 (18.8%) were the dominant structures.

Citations

Jan 14, 2011·Bioscience, Biotechnology, and Biochemistry·Mariko KimuraYoshinobu Kimura
Oct 30, 2015·Carbohydrate Research·Risa HoriuchiNobumitsu Miyanishi
Oct 31, 2000·Bioscience, Biotechnology, and Biochemistry·Y Kimura, E Kitahara
Mar 3, 1999·Bioscience, Biotechnology, and Biochemistry·N Q PalacpacT Seki
Aug 15, 2019·Biological Reviews of the Cambridge Philosophical Society·Harry C TjondroMorten Thaysen-Andersen
Jan 25, 2005·Bioscience, Biotechnology, and Biochemistry·Yoshinobu KimuraKosuke Kino

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