Structural elucidation of the polysaccharide moiety of a glycopeptide (GLPCW-II) from Ganoderma lucidum fruiting bodies

Carbohydrate Research
LiBin YeYingJie Pan

Abstract

A water-soluble glycopeptide (GLPCW-II) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE-Sepharose Fast-Flow and Sephacryl S-300 High Resolution Chromatography. The glycopeptide had a molecular weight of 1.2x10(4)Da (determined by HPLC), and consisted of approximately 90% carbohydrate and approximately 8% protein as determined using the phenol-sulfuric acid method and the BCA protein assay reagent kit, respectively. The polysaccharide moiety was composed mainly of D-Glc, L-Fuc, and D-Gal in the ratio of 1.00:1.09:4.09. To facilitate structure-activity studies, the structure of the GLPCW-II polysaccharide moiety was elucidated using 1H and 13C NMR spectroscopy including COSY, TOCSY, HMBC, HSQC, and ROESY, combined with GC-MS of methylated derivatives, and shown to consist of repeating units with the following structure: [Formula: see text].

References

Dec 1, 2006·Biochimica Et Biophysica Acta·Hildegard Geyer, Rudolf Geyer

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Citations

Mar 4, 2014·International Journal of Biological Macromolecules·K R SugumaranD Gowdhaman
Mar 1, 2012·Journal of Agricultural and Food Chemistry·Chia-Che TsaiShih-Hsiung Wu
Sep 24, 2011·Journal of Natural Products·James J La ClairMichael D Burkart
Jan 9, 2009·Bioscience, Biotechnology, and Biochemistry·Yan YangQingjiu Tang
Nov 18, 2015·Carbohydrate Polymers·Andrea C RuthesMarcello Iacomini
Nov 15, 2011·Natural Product Research·Guanglei Song, Qizhen Du
Aug 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shih-Fen LiaoChi-Huey Wong
Jun 14, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qiong WangZhongyang Ding
Jan 17, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing XieShao-Ping Li
Jan 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhiliang HeiJingfeng Wang
Sep 10, 2019·International Journal of Biological Macromolecules·Youli FuKan Ding
Dec 19, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Fahmi ShaherShaobo Zhou
Jul 25, 2017·Carbohydrate Polymers·Sugumaran K R, Ponnusami V

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