Structural evidence for a latch mechanism regulating access to the active site of SufS-family cysteine desulfurases.

Acta Crystallographica. Section D, Structural Biology
Jack A DunklePatrick A Frantom

Abstract

Cysteine serves as the sulfur source for the biosynthesis of Fe-S clusters and thio-cofactors, molecules that are required for core metabolic processes in all organisms. Therefore, cysteine desulfurases, which mobilize sulfur for its incorporation into thio-cofactors by cleaving the Cα-S bond of cysteine, are ubiquitous in nature. SufS, a type 2 cysteine desulfurase that is present in plants and microorganisms, mobilizes sulfur from cysteine to the transpersulfurase SufE to initiate Fe-S biosynthesis. Here, a 1.5 Å resolution X-ray crystal structure of the Escherichia coli SufS homodimer is reported which adopts a state in which the two monomers are rotated relative to their resting state, displacing a β-hairpin from its typical position blocking transpersulfurase access to the SufS active site. A global structure and sequence analysis of SufS family members indicates that the active-site β-hairpin is likely to require adjacent structural elements to function as a β-latch regulating access to the SufS active site.

References

Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L ZhengD R Dean
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·K KobayashiN Ogasawara
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Douglas Van HoewykElizabeth A H Pilon-Smits
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
May 1, 2012·Bioinformatics·Douglas L Theobald, Phillip A Steindel
May 24, 2012·Biochemical Society Transactions·Tadhg P BegleyFred W McLafferty
Sep 10, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Thomas RoretClaude Didierjean
Sep 1, 2008·EcoSal Plus·Nicholas M Kredich
Mar 10, 2016·Acta Crystallographica. Section D, Structural Biology·Vincent OliericMeitian Wang
Mar 23, 2017·Biochemistry·Martin BühningSilke Leimkühler
Nov 2, 2018·Biochemical Society Transactions·Chenkang Zheng, Patricia C Dos Santos
Jul 5, 2019·The Journal of Biological Chemistry·Süleyman GünalJonathan Wolf Mueller
Sep 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Shachin Patra, David P Barondeau
Oct 8, 2019·Acta Crystallographica. Section D, Structural Biology·Dorothee LiebschnerPaul D Adams

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Citations

Nov 21, 2020·Journal of the Science of Food and Agriculture·Saheed Adekunle AkinolaOlubukola Oluranti Babalola

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