PMID: 109509Apr 1, 1979

Structural evidence for a polymorphic or allelic form of the heavy chain variable region

The Journal of Immunology : Official Journal of the American Association of Immunologists
J L Claflin, S Rudikoff

Abstract

The heavy (H) chains of anti-phosphocholine (PC) antibodies from C57BL/6J and CBA/J were sequenced through the N-terminal 36 residues and compared with previously published sequences of A/J anti-PC antibody and BALB/c PC-binding myeloma proteins T15, M603, and M511. Each of these antibody preparations contained molecules having light (L) chains and idiotypic determinants of T15, M511, and M603 indicating the presence of at least three different anti-PC antibodies in each pool. The structures of the C57BL/6J and CBA/J H chains each revealed a single sequence from positions 1 to 36 (which includes the first complementarity determining region (CDR), and they were identical. The first CDR was identical to that previously found for BALB/c and A/J indicating that this portion of these antibody molecules is highly conserved throughout inbred mice and is probably critical to PC-binding. A surprising finding was that both C57NL/6 and CBA sequence differed from the BALB/c and A/J sequences at two positions, residue 14 and 16. Since each of these strains differs at the allotype locus, the data indicates that the evolution of allotypy in mice occurred after variable region diversity for the particular genes.

Related Concepts

Alleles
Binding Sites, Antibody
IgM2
Immunoelectrophoresis
Framework Region, Immunoglobulin
Immunoglobulin Heavy Chain Subgroup VH-III
Mice, Inbred CBA
Mice, Inbred C57BL
Phosphorylcholine
Genetic Polymorphism

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