Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY

The EMBO Journal
Osman MirzaH Ronald Kaback

Abstract

Cation-coupled active transport is an essential cellular process found ubiquitously in all living organisms. Here, we present two novel ligand-free X-ray structures of the lactose permease (LacY) of Escherichia coli determined at acidic and neutral pH, and propose a model for the mechanism of coupling between lactose and H+ translocation. No sugar-binding site is observed in the absence of ligand, and deprotonation of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the initial step in H+ transduction.

References

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Citations

Dec 17, 2010·The Journal of Membrane Biology·H Ronald KabackYonggang Zhou
Dec 21, 2006·Biochemistry·Irina N SmirnovaH Ronald Kaback
Oct 15, 2011·Biochemistry·Irina SmirnovaH Ronald Kaback
Nov 24, 2011·Biochemistry·Junichi SugiharaH Ronald Kaback
Aug 20, 2009·Biochemistry·Irina SmirnovaH Ronald Kaback
Sep 2, 2009·Biochemistry·Lan Guan, H Ronald Kaback
May 15, 2013·Nature·Hongjin ZhengTamir Gonen
Jun 7, 2013·Nature Structural & Molecular Biology·Peter J F Henderson, Stephen A Baldwin
Mar 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yonggang ZhouH Ronald Kaback
Apr 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Juan J Garcia-CelmaKlaus Fendler
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Mohammad S Yousef, Lan Guan
Dec 5, 2009·Proceedings of the National Academy of Sciences of the United States of America·Irina SmirnovaH Ronald Kaback
May 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yiling Nie, H Ronald Kaback
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lukasz KowalczykManuel Palacín
May 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Vincent ChaptalJeff Abramson
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Irina SmirnovaH Ronald Kaback
Feb 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xiaoxu JiangH Ronald Kaback
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yonggang ZhouH Ronald Kaback
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·M Gregor MadejH Ronald Kaback
Aug 30, 2012·Proceedings of the National Academy of Sciences of the United States of America·Junichi SugiharaH Ronald Kaback
Oct 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Irina SmirnovaH Ronald Kaback
Jul 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·David EwersChristoph Fahlke
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shintaro DokiOsamu Nureki
Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·M Gregor MadejH Ronald Kaback
May 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Irina SmirnovaH Ronald Kaback
Nov 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·M Gregor Madej, H Ronald Kaback
Dec 15, 2010·The Journal of Biological Chemistry·Lan GuanSiva P Ankeshwarapu
Jul 7, 2011·The Journal of Biological Chemistry·Yonggang ZhouH Ronald Kaback
Sep 28, 2006·The Journal of Biological Chemistry·Yiling NieH Ronald Kaback
Aug 7, 2012·Journal of Bacteriology·S Vivek Jakkula, Lan Guan
Apr 2, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Nicholas M Burton, Lesley J Bruce
Nov 23, 2012·BMC Biochemistry·Ye Sun, John M Ward
May 7, 2011·BMC Bioinformatics·Damien PauletChristophe Béroud
Nov 10, 2013·PloS One·Phaneendra Kumar DuddempudiMichaela Jansen
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Devdoot S MajumdarH Ronald Kaback
Sep 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lan GuanH Ronald Kaback
Nov 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yoav AdamShimon Schuldiner
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·M Gregor MadejH Ronald Kaback
Dec 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·H Ronald KabackNatalia Ermolova
Oct 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Irina SmirnovaH Ronald Kaback
Apr 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tetiana SerdiukDaniel J Müller
May 10, 2013·Annual Review of Biophysics·Yigong Shi
May 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Irina SmirnovaH Ronald Kaback

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