PMID: 9556214Apr 29, 1998Paper

Structural factors affect the interactions of anticardiotoxin antibodies and cobra venom cardiotoxins

Biochemistry and Molecular Biology International
L S ChangS R Lin

Abstract

Two antibody preparations against cardiotoxins were raised by immunizing rabbits with cardiotoxin 1 and cardiotoxin 3, respectively. The two antibody preparations showed precipitin reactions with cardiotoxins 1, 2, 3 and 5, respectively. However, the results of competitive enzyme-linked immunoassay revealed that the respective cardiotoxin molecules exhibited different reactivity toward anticardiotoxin antibodies. Moreover, the order of reactivity with antibodies was not in line with the degree of their sequence identity. This suggest that the anticardiotoxin antibodies may recognize conformational epitopes rather than sequential ones in the toxin molecules. Alternatively, the four cardiotoxins reacted well with the antibodies in the absence of competitor, suggesting that sequence variations with cardiotoxin molecules may not exclusively influence the potential use of the anticardiotoxin antibodies for the neutralization of the activity of cardiotoxin variants.

Citations

Mar 10, 2000·Toxicon : Official Journal of the International Society on Toxinology·L S ChangS R Lin
Mar 3, 2010·Toxicon : Official Journal of the International Society on Toxinology·Pei-Hsiu KaoLong-Sen Chang
May 18, 2011·Toxicon : Official Journal of the International Society on Toxinology·Li-Wen ChenLong-Sen Chang

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