Structural feature of the major but not cytokine-inducing molecular species of lipoteichoic acid

Journal of Biochemistry
M HashimotoS Kusumoto

Abstract

Previously, lipoteichoic acid (LTA) of Enterococcus hirae was found to exhibit definite cytokine-inducing activity but synthetic specimens which share the fundamental structural principles proposed for LTA had no corresponding activity. We also showed recently that several minor components totally less than 5% of the LTA fraction from E. hirae ATCC 9790 possessed the activity, whereas the major component (over 90%) did not [Suda, Y., Tochio, H., Kawano, K., Takada, H., Yoshida, T., Kotani, S., and Kusumoto, S. (1995) FEMS Immun. Med. Microbiol. 12, 97-112]. In the present study, the structure of the major component of LTA was studied in an attempt to elucidate the reason for the lack of the activity in the synthetic compounds. The major component of the LTA was first digested by hydrofluoric acid hydrolysis to cleave phosphodiester linkages present. The hydrolysis products were separated and characterized by means of NMR and MS. The linkage positions of the original phosphodiesters were determined from the NMR spectra of an alkali-treated product without hydrofluoric acid degradation. The compound was proved to consist of 1,3-linked poly(glycerophosphate) and a lipid anchor, Glc(alpha1-2)Glc(alpha1-3)acyl(2)Gro, the former bein...Continue Reading

Citations

Feb 17, 2009·The Journal of Biological Chemistry·Kazuki TawaratsumidaMasahito Hashimoto
Feb 7, 2001·The Journal of Experimental Medicine·S MorathT Hartung
Oct 29, 2008·Antimicrobial Agents and Chemotherapy·Hemamali J WarshakoonSunil A David
Feb 7, 2007·Infection and Immunity·Masahito HashimotoYasuo Suda
Jan 18, 2002·Infection and Immunity·Siegfried MorathThomas Hartung
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Francis C Neuhaus, James Baddiley
Oct 30, 2007·Biochemical and Biophysical Research Communications·Simon J FinneyAnne Burke-Gaffney
Aug 12, 2016·Microbiology and Molecular Biology Reviews : MMBR·Minh Thu Nguyen, Friedrich Götz
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philipp HennekeDouglas T Golenbock
Apr 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·David L HastyItzhak Ofek
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Masahito HashimotoFriedrich Götz
Dec 24, 2005·International Immunology·Masahito HashimotoYasuo Suda

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