Structural features in the 3' external transcribed spacer affecting intragenic processing of yeast rRNA

Journal of Molecular Biology
J HitchenRoss N Nazar

Abstract

A highly conserved extended hairpin structure in the 3' external transcribed spacer (3' ETS) region of nascent eukaryotic rRNA transcripts is essential for the maturation of the large ribosomal subunit RNAs (5.8 S and 25 to 28 S rRNAs). Systematic changes were introduced into this structure by PCR-mediated mutagenesis and the mutant rDNAs were expressed in vivo to determine the structural features that are essential for rRNA maturation. Changes in the lower half of the stem or the large loop at the end had little or no effect on the maturation of either the 5.8 S or 25 S rRNA, but changes that disrupted secondary structure in the upper half of this stem had equal and dramatic effects on both RNAs. When the RNA stem was incubated with a cellular protein extract, gel retardation studies indicated that the stem forms a ribonucleoprotein complex, and a comparison with mutant RNA indicated that protein binding could be compromised by changes that were critical for rRNA maturation. Sequence comparisons with other spacer regions as well as snRNAs reveal some structural analogy, which, when taken together with the mutational studies, raise the possibility that this hairpin functions during RNA processing in a manner that may be analogo...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 25, 1992·Nucleic Acids Research·L Good, R N Nazar
Feb 20, 1992·Journal of Molecular Biology·C A van der SandeR J Planta
Jan 1, 1990·Methods in Enzymology·S M Jazwinski
Feb 1, 1989·Molecular and Cellular Biology·W MustersR J Planta
Jan 1, 1987·Methods in Enzymology·L Hennighausen, H Lubon
May 23, 1969·Science·O L Miller, B R Beatty
Nov 24, 1995·The Journal of Biological Chemistry·Y LeeR N Nazar
Jan 1, 1995·Annual Review of Biochemistry·E S Maxwell, M J Fournier
Jun 3, 1994·Journal of Molecular Biology·Y F MelekhovetsR N Nazar
Apr 1, 1993·Molecular and Cellular Biology·J P Morrissey, D Tollervey
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·W Q Liang, M J Fournier
Feb 12, 1998·Journal of Molecular Biology·L GoodR N Nazar

❮ Previous
Next ❯

Citations

Mar 18, 2000·Antimicrobial Agents and Chemotherapy·K E Miletti, M J Leibowitz
Jan 19, 2016·Translation·Michael Gamalinda, John L Woolford
Feb 26, 2000·Annual Review of Genetics·J Venema, D Tollervey
Mar 30, 2001·The Journal of Biological Chemistry·A I Lalev, R N Nazar
Jul 19, 2002·FEBS Letters·Xinyu Song, Ross N Nazar
Oct 21, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sadeq Nabavi, Ross N Nazar
Mar 30, 2002·The Journal of Biological Chemistry·Priyanka D AbeyrathneRoss N Nazar
Jan 8, 2017·The Biochemical Journal·Salini Konikkat, John L Woolford
Mar 7, 2008·The Journal of Biological Chemistry·Sadeq Nabavi, Ross N Nazar
May 26, 1998·Journal of Molecular Biology·C Allmang, D Tollervey
Jan 23, 2014·Genes & Development·Michael GamalindaJohn L Woolford

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.