PMID: 6162151Nov 25, 1980Paper

Structural features of Bacillus precursor 5S RNA involved in the interaction with RNAase M5

Nucleic Acids Research
W J StiekemaR J Planta

Abstract

Mature 5S (m5S) RNA from Bacillus licheniformis specifically and almost completely inhibits in vitro maturation of bacillus precursor 5S (p5S) RNA, showing that the maturation enzyme RNAase M5 can recognize Bacillus m5S RNA. E. coli m5S RNA is a much less efficient inhibitor, whereas S. carlsbergensis 5S RNA inhibits maturation by about 70%. The differences in inhibition can be correlated with the position of the sequence UAGG (residues 101-104 in B. licheniformis m5S RNA) relative to the double-helical region formed by the 5'- and 3'-terminal sequences (molecular stalk) of m5S RNA. Recent experiments by Meyhack and Pace (Biochemistry 17 (1980) 5804-5810) demonstrated this UAGG sequence to be indispensable for processing of p5S RNA. Other elements of secondary and/or tertiary structure are also required, however. The effect of artificially constructed "5S RNA" molecules having defined disturbances in the base-pairing within the molecular stalk on in vitro maturation shows that base-pairing in the immediate neighbourhood of the bonds to be cleaved during maturation is crucial to recognition of p5S RNA by RNAase M5. G.U pairs are tolerated in this region, however, without loss of efficiency in maturation. Base-pairing does not ha...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B MeyhackN R Pace
Jan 1, 1979·Annual Review of Biochemistry·J Abelson
Sep 1, 1979·Cell·C L PeeblesJ Abelson
Nov 14, 1977·Molecular & General Genetics : MGG·H A RauéR J Planta
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A L BothwellS Altman
Jan 1, 1976·Annual Review of Biochemistry·R P Perry
Aug 7, 1975·Nature·G E Fox, C R Woese
Nov 1, 1975·European Journal of Biochemistry·H A RauéR J Planta
Apr 1, 1980·Cell·R KoleS Altman
May 24, 1980·Nucleic Acids Research·W J StiekemaR J Planta
Jan 11, 1980·Nucleic Acids Research·V A Erdmann

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