Structural Features of DNA G-Quadruplexes Revealed by Surface-Enhanced Raman Spectroscopy

The Journal of Physical Chemistry Letters
Yang LiXinhua Guo

Abstract

Surface-enhanced Raman spectroscopy (SERS) has been successfully used for the label-free detection of single-stranded oligonucleotides. However, the detection of complex DNA secondary structures remains a challenge. Structural features of diverse DNA G-quadruplexes were investigated via a novel SERS method. As a result, a series of highly reproducible and sensitive SERS signatures featuring the structures of G-quadruplexes were obtained. For the first time, we reported remarkably enhanced SERS bands corresponding to purine ring breathing vibrations. Moreover, we observed that by measuring the intensity of the bands corresponding to intramolecular hydrogen bonds, we could quantitatively assess the stability of the G-quadruplexes. Because no labels on DNA strands were present as the experiments were carried out in the solution, the fingerprint peaks reflect the native, internal structure of the G-quadruplexes accurately. The method here detailed provides new insights into the promising applications of diverse DNA structural studies.

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Citations

May 24, 2019·Analytical and Bioanalytical Chemistry·Francesco PapiSnežana Miljanić
May 7, 2020·Chembiochem : a European Journal of Chemical Biology·David A PriceSean D Moran
Jan 31, 2021·Nanomaterials·Luca Guerrini, Ramon A Alvarez-Puebla
Feb 13, 2021·International Journal of Biological Macromolecules·Xiaoxuan XiangXinhua Guo
Mar 28, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mengyu JingXiumin Shi
Aug 7, 2021·Biosensors·Irene CalderonRamon A Alvarez-Puebla
Aug 8, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Andrei StefancuCiprian Tomuleasa
Sep 23, 2020·Analytical Chemistry·Dan LiYang Li
Aug 14, 2020·The Journal of Physical Chemistry Letters·Miguel Martínez-CalvoJosé L Mascareñas
May 16, 2019·The Journal of Physical Chemistry Letters·Yang LiXinhua Guo

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