PMID: 7525737Nov 1, 1994Paper

Structural features of keratin intermediate filaments

The Journal of Investigative Dermatology
P M SteinertD A Parry

Abstract

The first step in the assembly of a keratin intermediate filament (KIF) is the formation of a type I/type II heterodimer molecule in which two chains become aligned in parallel and close axial registration to form a flexible segmented alpha-helical coiled-coil rope 46 nm long. The segments of coiled-coil are interspersed by sequences that introduce irregularities of unknown structure. Here we have modeled two of these, the link L2 and the heptad discontinuity located near the middle of segment 2B. In a model for L2, the orientation of the coiled-coil structure is turned through about 180 degrees over the eight residue stretch constituting this link segment. In contrast, the heptad discontinuity in segment 2B would seem to result in only minimal distortion of the coiled-coil rope, contrary to previous expectations. Little is known about how the neighboring molecules are aligned and packed within the assembled KIF. Crosslinking experiments with KIF have determined that two neighboring molecules are aligned anti-parallel and axially in three ways, and predict that similarly-directed molecules could be overlapped by about 1 nm. The two-dimensional surface lattice resulting from these data predicts an axial periodicity of 22.6 nm, w...Continue Reading

References

Dec 15, 1976·Journal of Molecular Biology·P M SteinertS B Zimmerman
Jan 1, 1992·International Review of Cytology·K Albers, E Fuchs
Jul 1, 1992·The Journal of Cell Biology·K L VikstromG G Borisy
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R D FraserE Suzuki
Mar 29, 1985·Biochemical and Biophysical Research Communications·D A ParryP M Steinert
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Jan 1, 1994·The Journal of Investigative Dermatology·J A RothnagelD R Roop
Jan 1, 1994·The Journal of Investigative Dermatology·J M YangJ G Compton
Jan 1, 1994·The Journal of Investigative Dermatology·W H McLeanS M Morley
Jun 1, 1993·The Journal of Investigative Dermatology·P M Steinert
Aug 1, 1993·The Journal of Investigative Dermatology·K StephensA Spencer

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Citations

Sep 9, 2005·DNA Sequence : the Journal of DNA Sequencing and Mapping·J MinorK Credille
May 30, 1997·Biochimica Et Biophysica Acta·K Noben-TrauthP M Nishina
Jul 1, 1997·The International Journal of Biochemistry & Cell Biology·A WaseemN H Waseem
Sep 26, 1997·Journal of Dermatological Science·M ManabeH Ogawa

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