Structural features of nephritogenic lupus autoantibodies

Methods : a Companion to Methods in Enzymology
M T VargasM P Madaio

Abstract

We have identified monoclonal antibodies derived from MRL-lpr/lpr lupus-prone mice that produced nephritis after passive transfer to normal mice. Our present goal was to elucidate the structural and immunochemical features of nephritogenic Ig that facilitate immune deposition. For this purpose the antigen binding properties, capacity to form immune deposits, and nucleotide sequence of a genetically related autoantibody subgroup were compared. The prototype, H147 (an IgG encoded by 7183/81X VH gene), produced glomerular and tubular basement membrane, mesangial immune deposits, and proliferative glomerulonephritis after passive transfer to normal mice. For comparison three other 7183/81X encoded anti-DNA IgG (H257, H171, and H8a) were evaluated (predicted heavy chain aa homology >75%). H257 produced similar types of immune deposits as H147, and this was associated with nephritis; H8a produced predominantly mesangial deposits, whereas H171 did not produce significant deposits. Although their antigen binding profile to a panel of soluble autoantigens was variable, only H147 and H257 bound to both mesangial and aortic endothelial cell surfaces. V gene sequence analysis of the IgG suggests that individual residues, motifs, and confor...Continue Reading

Citations

Feb 6, 2004·The Journal of Experimental Medicine·Zhiyan LiangChandra Mohan
Oct 26, 2006·Arthritis Research & Therapy·Andrea Becker-MerokJohannes C Nossent
Feb 28, 1998·Immunologic Research·M P Madaio
Jun 30, 2012·Kidney International·Michael P Madaio
Feb 28, 2009·Seminars in Arthritis and Rheumatism·Luciana BredaFrancesco Chiarelli
Apr 19, 2015·Autoimmunity Reviews·Alice BonanniGian Marco Ghiggeri
May 3, 2014·Journal of the American Society of Nephrology : JASN·Maurizio BruschiGian Marco Ghiggeri
Oct 8, 1999·Pharmacology & Therapeutics·N B Blatt, G D Glick

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.