Structural, functional, and mutational analysis of the NblA protein provides insight into possible modes of interaction with the phycobilisome.

The Journal of Biological Chemistry
Monica DinesNoam Adir

Abstract

The enormous macromolecular phycobilisome antenna complex (>4 MDa) in cyanobacteria and red algae undergoes controlled degradation during certain forms of nutrient starvation. The NblA protein (approximately 6 kDa) has been identified as an essential component in this process. We have used structural, biochemical, and genetic methods to obtain molecular details on the mode of action of the NblA protein. We have determined the three-dimensional structure of the NblA protein from both the thermophilic cyanobacterium Thermosynechococcus vulcanus and the mesophilic cyanobacterium Synechococcus elongatus sp. PCC 7942. The NblA monomer has a helix-loop-helix motif which dimerizes into an open, four-helical bundle, identical to the previously determined NblA structure from Anabaena. Previous studies indicated that mutations to NblA residues near the C terminus impaired its binding to phycobilisome proteins in vitro, whereas the only mutation known to affect NblA function in vivo is located near the protein N terminus. We performed random mutagenesis of the S. elongatus nblA gene which enabled the identification of four additional amino acids crucial for NblA function in vivo. This data shows that essential amino acids are not confined...Continue Reading

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Citations

Mar 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Dvir HarrisNoam Adir
May 7, 2014·The Plant Journal : for Cell and Molecular Biology·Eleonora SenderskyRakefet Schwarz
Jul 16, 2015·The Plant Journal : for Cell and Molecular Biology·Eleonora SenderskyRakefet Schwarz
Dec 4, 2012·Biochimica Et Biophysica Acta·Ailie Marx, Noam Adir
Mar 27, 2018·The Plant Journal : for Cell and Molecular Biology·Mali LeviRakefet Schwarz
Nov 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Cheng ZhaoKai-Hong Zhao
Oct 16, 2018·Environmental Microbiology·Karl Forchhammer, Rakefet Schwarz
Oct 11, 2012·Microbes and Environments·Yukari Yoshida-TakashimaTakashi Yoshida
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