Structural genes for Mg-chelatase subunits in barley: Xantha-f, -g and -h

Molecular & General Genetics : MGG
P E JensenK W Henningsen

Abstract

Barley mutants in the loci Xantha-f, Xantha-g and Xantha-h, when fed with 5-aminolevulinate in the dark, accumulate protoporphyrin IX. Mutant alleles at these loci that are completely blocked in protochlorophyllide synthesis are also blocked in development of prolamellar bodies in etioplasts. In contrast to wild type, the xan-f, -g and -h mutants had no detectable Mg-chelatase activity, whereas they all had methyltransferase activity for synthesis of Mg-protoporphyrin monomethyl ester. Antibodies recognising the CH42 protein of Arabidopsis thaliana and the OLIVE (OLI) protein of Antirrhinum majus immunoreacted in wild-type barley with 42 and 150 kDa proteins, respectively. The xan-h mutants lacked the protein reacting with antibodies raised against the CH42 protein. Two xan-f mutants lacked the 150 kDa protein recognised by the anti-OLI antibody. Barley genes homologous to the A. majus olive and the A. thaliana Ch-42 genes were cloned using PCR and screening of cDNA and genomic libraries. Probes for these genes were applied to Northern blots of RNA from the xantha mutants and confirmed the results of the Western analysis. The mutants xan-f27, -f40, -h56 and -h57 are defective in transcript accumulation while -h38 is defective i...Continue Reading

References

Feb 1, 1979·Archives of Biochemistry and Biophysics·P A CastelfrancoB E Wezelman
Apr 1, 1989·European Journal of Biochemistry·G von HeijneR G Herrmann
Jul 1, 1985·FEBS Letters·W Möller, R Amons
Apr 1, 1988·Trends in Biochemical Sciences·C G KannangaraD von Wettstein
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·R HöfgenD von Wettstein
Apr 15, 1994·Journal of Molecular Biology·D W BollivarC E Bauer
Apr 1, 1994·The Biochemical Journal·C J Walker, J D Weinstein
Oct 1, 1993·Plant Molecular Biology·O MadsenK A Marcker
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·O OlsenD von Wettstein
Jan 15, 1996·European Journal of Biochemistry·R D WillowsD von Wettstein

❮ Previous
Next ❯

Citations

Feb 8, 2006·Plant Molecular Biology·Ruairidh J H SawersThomas P Brutnell
Jun 14, 2005·Trends in Plant Science·Samuel I Beale
Apr 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Eric HosyHervé Sentenac
Oct 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·A HanssonM Hansson
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·M Hansson, C G Kannangara
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·A HanssonM Hansson
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·S GräfeF Hänel
May 22, 2004·European Journal of Biochemistry·Vanessa LakeMats Hansson
Aug 5, 2003·Genome Génome / Conseil National De Recherches Canada·Xiaolei WuN Jerry Chatterton
Mar 13, 2014·Plant Physiology and Biochemistry : PPB·Ilka BraumannMats Hansson
Jul 21, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Meiling GaoYiqun Weng
Oct 26, 2005·Plant Molecular Biology·Suchi SoodBaishnab C Tripathy
Dec 3, 2014·G3 : Genes - Genomes - Genetics·Benjamin W CampbellRobert M Stupar
Mar 11, 2015·Plant Biotechnology Journal·Dilrukshi S K NagahatennaRyan Whitford

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
A HanssonMats Hansson
Proceedings of the National Academy of Sciences of the United States of America
Mats Hansson, C G Kannangara
Proceedings of the National Academy of Sciences of the United States of America
S GräfeF Hänel
© 2021 Meta ULC. All rights reserved