Structural homologies between two HLA B27-restricted peptides suggest residues important for interaction with HLA B27

International Immunology
S HuetA J McMichael

Abstract

Recently we described an HLA B27-restricted peptide derived from HIV gag p24 protein. In this study we have isolated an HLA B27-restricted peptide from the nucleoprotein (NP) of influenza A virus. The shortest fragment recognized by cytotoxic T lymphocyte (CTL) is eight amino acids long, residues 384-391. Comparison of the sequence of these two HLA B27 restricted peptides reveals homologies which can be aligned from one peptide to the other. Of the eight residues, two are identical: tryptophan and isoleucine. Both peptides have a positively charged residue at the N terminus, lysine at position 265 of gag and arginine at position 384 of NP. Using modified peptides we have shown that lysine or arginine is crucial for the interaction with HLA B27. The wild-type gag peptide blocked CTL recognition of NP peptide by influenza-specific CTL, but removal of the lysine prevented inhibition of NP peptide recognition. The importance of these charged residues was confirmed by the observation that truncated NP and gag peptides where the lysine or arginine was removed were not recognized by specific CTL. Further studies showed that the tryptophan residue influenced the association of the gag peptide with HLA B27, because the affinity of the g...Continue Reading

Citations

Oct 20, 1991·Biotechnology and Bioengineering·D NeedhamR Tran-Son-Tay
Jan 1, 1995·Immunogenetics·H G RammenseeS Stevanoviíc
Jan 1, 1992·Rheumatology International·H Kellner, D Yu
Jun 1, 1991·Research in Immunology·A McMichael, J Bell
Feb 1, 1992·Current Opinion in Immunology·A Sette, H M Grey
Feb 1, 1994·Current Opinion in Immunology·V H Engelhard
May 5, 2009·Nature Immunology·Stefan TenzerAstrid K N Iversen
Feb 14, 2002·Scandinavian Journal of Immunology·A C M BoonA D M E Osterhaus
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Ohno
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R A ColbertJ A Frelinger
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·D RognanA Daser
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·F BoisgeraultA Toubert
Jan 1, 1991·The Journal of Experimental Medicine·P A Robbins, A J McMichael
Feb 1, 1992·The Journal of Experimental Medicine·L PazmanyA McMichael
Apr 20, 2012·Rheumatology·Kirsty McHugh, Paul Bowness
Apr 27, 2007·Southern Medical Journal·Mesut Ogrendik
Nov 22, 2012·Viruses·Carolien E van de SandtGuus F Rimmelzwaan
Apr 12, 2015·Annual Review of Immunology·Paul Bowness
Dec 1, 1993·Current Biology : CB·T ElliottA McMichael
Jan 1, 1990·Scandinavian Journal of Rheumatology. Supplement·P Ivanyi
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·T P Levine, B M Chain
Sep 26, 1991·Nature·T S JardetzkyD C Wiley

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