Structural homology among four nodaviruses as deduced by sequencing and X-ray crystallography

Journal of Molecular Biology
P KaesbergJ E Johnson

Abstract

The genomic RNA2s of nodaviruses encode a single gene, that of protein alpha, the precursor of virion proteins beta and gamma. We compared the sequences of the RNA2s of the nodaviruses, black beetle virus (BBV), flock house virus, boolarra virus and nodamura virus, with the objective of identifying homologies in the primary and secondary structure of these RNAs and in the structure of their encoded protein. The sequences of the four RNAs were found to be similar, so that homologous regions relating to translation and RNA replication were readily identified. However, the overall, secondary structures in solution, deduced from calculations of optimal Watson-Crick base-pairing configurations, were very different for the four RNAs. We conclude that a particular, overall, secondary structure in solution within host cells is not required for virus viability. The partially refined X-ray structure of BBV (R = 26.4% for the current model) was used as a framework for comparing the structure of the encoded proteins of the four viruses. Mapping of the four protein sequences onto the BBV capsid showed many amino acid differences on the outer surface, indicating that the exteriors of the four virions are substantially different. Mapping in t...Continue Reading

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Citations

Apr 19, 2005·European Biophysics Journal : EBJ·Andreas Hinz, Hans-Joachim Galla
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S X WuP Kaesberg
Aug 10, 2007·Journal of Virology·Tian-Cheng LiNaokazu Takeda
Mar 5, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Pitchanee Jariyapong
Jul 18, 2001·The Journal of General Virology·K N JohnsonL A Ball
Aug 20, 2002·The Journal of General Virology·John V Krondiris, Diamantis C Sideris
Jan 30, 2019·Journal of Fish Diseases·Young Chul KimHyun Do Jeong
Dec 2, 1999·The Journal of General Virology·T Nagai, T Nishizawa
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Mar 22, 2021·The Journal of Biological Chemistry·John E Johnson, Arthur J Olson
Jun 1, 1993·Journal of Virology·L A Ball, Y Li
Apr 29, 2006·Virus Research·Chuanfeng LiuYuanyang Hu

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