Structural Illumination of Equine MHC Class I Molecules Highlights Unconventional Epitope Presentation Manner That Is Evolved in Equine Leukocyte Antigen Alleles

The Journal of Immunology : Official Journal of the American Association of Immunologists
Shugang YaoChun Xia

Abstract

MHC class I (MHC I)-restricted virus-specific CTLs are implicated as critical components in the control of this naturally occurring lentivirus and in the protective immune response to the successfully applied attenuated equine infectious anemia virus vaccine in the horse. Nevertheless, the structural basis for how the equine MHC I presents epitope peptides remains unknown. In this study, we investigated the binding of several equine infectious anemia virus-derived epitope peptides by the ability to refold recombinant molecules and by thermal stability, and then by determining the x-ray structure of five peptide-MHC I complexes: equine MHC class I allele (Eqca)-N*00602/Env-RW12, Eqca-N*00602/Gag-GW12, Eqca-N*00602/Rev-QW11, Eqca-N*00602/Gag-CF9, and Eqca-N*00601/Gag-GW12. Although Eqca-N*00601 and Eqca-N*00602 differ by a single amino acid, Eqca-N*00601 exhibited a drastically different peptide presentation when binding a similar CTL epitope, Gag-GW12; the result makes the previously reported function clear to be non-cross-recognition between these two alleles. The structures plus Eqca-N*00602 complexed with a 9-mer peptide are particularly noteworthy in that we illuminated differences in apparent flexibility in the center of th...Continue Reading

References

May 20, 1991·Journal of Molecular Biology·M A SaperD C Wiley
Jan 11, 1990·Nucleic Acids Research·T C McGuireK I O'Rourke
Feb 1, 1989·Current Opinion in Immunology·O Narayan
Jan 1, 1985·Reviews of Infectious Diseases·W P Cheevers, T C McGuire
May 1, 1994·Virus Research·D C SellonT C McGuire
Sep 4, 1999·Journal of Molecular Evolution·E C Holmes, S A Ellis
Mar 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·S A MiguelesM Connors
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsThomas C Terwilliger
Dec 3, 2002·Science·Ilhem MessaoudiJanko Nikolich-Zugich
Dec 31, 2002·Immunology Letters·Toranosuke TobitaHaruo Kozono
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Travis C McGuireDavid J Prieur
Dec 17, 2003·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·C ChungT C McGuire
Jan 22, 2004·The Journal of Experimental Medicine·Martin HülsmeyerBarbara Uchanska-Ziegler
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lenong Li, Marlene Bouvier
Jul 9, 2004·Veterinary Research·Caroline LerouxRonald C Montelaro
Jul 24, 2004·Acta Crystallographica. Section D, Biological Crystallography·Minghai ZhouGeorge F Gao
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Jun 14, 2005·Molecular Immunology·Lenong Li, Marlene Bouvier
Oct 13, 2005·Immunogenetics·Rebecca L TallmadgeDouglas F Antczak
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Fuliang ChuGeorge F Gao
Jun 1, 2007·Journal of Virology·John T LoffredoDavid I Watkins
Dec 28, 2007·The New England Journal of Medicine·Robert Steinbrook
Nov 13, 2008·Immunogenetics·Ilka HoofMorten Nielsen
Dec 17, 2009·The Journal of Experimental Medicine·Jinghe HuangXu G Yu
Jan 26, 2010·Immunogenetics·Rebecca L TallmadgeDouglas F Antczak
Nov 6, 2010·Science·UNKNOWN International HIV Controllers StudyMeng Zhao

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Citations

Dec 3, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·William J LiuWenjie Tan
Oct 21, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin XiaoWilliam J Liu
Nov 30, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lizhen MaMax Chun Xia
Feb 1, 2017·Biological Chemistry·Tracy M JosephsStephanie Gras
Aug 12, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin XiaoGeorge F Gao
May 15, 2021·Developmental and Comparative Immunology·Yulu DuanChun Xia

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