Structural influence of mono and polyhydric alcohols on the stabilization of collagen

Colloids and Surfaces. B, Biointerfaces
R UshaT Ramasami

Abstract

In the present study, solvents effects on the structure of collagen have been examined by circular dichroism and their interfacial tension at glass/liquid and Teflon/liquid. Changes in the conformations of the protein have been analyzed after equilibration with aqueous solutions of monohydric and polyhydric alcohols like methanol, ethanol, n-propanol, propane-2-diol and glycerol. The results from viscosity and Circular dichroism (CD) spectra suggest a clear distinction in the structural changes for collagen with monohydric alcohols as against polyhydric ones. The surface tension and interfacial tension at glass (high surface energy, HFSE) and Teflon (Low surface energy, LSFE) reflect similar differences between the monohydric and polyhydric alcohols. Studies on the interfacial energy of the adsorbed protein at glass/solution interface compared to that of Teflon/solution interface show that the water structure near glass gets perturbed leading to an increase in the average free energy of the bulk water phase and a reduction in hydrophobic effect near the glass. The results suggest that the different solvents alter the hydrophobic effect on the hydrated protein to different extent and thus influence folding equilibrium of the pro...Continue Reading

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Citations

Sep 12, 2012·Journal of Biomolecular Structure & Dynamics·Yan LiuQiqing Zhang
Apr 3, 2012·Chemistry Central Journal·Lee G GonzalezTim J Wess
Dec 11, 2013·Biochimica Et Biophysica Acta·Shangping WangWillem F Wolkers
Nov 8, 2014·European Biophysics Journal : EBJ·Arun GopinathJonnalagadda Raghava Rao
Jan 11, 2011·International Journal of Biological Macromolecules·Jiheng Li, Guoying Li
Sep 22, 2009·Biomaterials·Nima SaeidiJeffrey W Ruberti
Oct 26, 2007·Colloids and Surfaces. B, Biointerfaces·R M T FernandesC W B Bezerra
Aug 7, 2014·Scientific Reports·Marimuthu NidhinBalachandran Unni Nair
Nov 20, 2013·Colloids and Surfaces. B, Biointerfaces·Laura PastorinoCarmelina Ruggiero
Dec 19, 2012·International Journal of Biological Macromolecules·Ganesh ShanmugamBalaraman Madhan
Dec 3, 2014·International Journal of Biological Macromolecules·Jiheng LiGuoying Li
May 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shichen ZhuYang Hu
Apr 23, 2020·Journal of Biomedical Materials Research. Part a·Zhenhua TianGuoying Li
Jun 22, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Annabell TenbollSilvia Mittler
Dec 22, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Jochen BürckMurat Kazanci

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