Structural Insight into Substrate Specificity of 3-Hydroxypropionyl-Coenzyme A Dehydratase from Metallosphaera sedula

Scientific Reports
Donghoon Lee, Kyung-Jin Kim

Abstract

Metallosphaera sedula is a thermoacidophilic autotrophic archaeon known to utilize the 3-hydroxypropionate/4-hydroxybutyrate cycle (3-HP/4-HB cycle) as carbon fixation pathway. 3-Hydroxypropionyl-CoA dehydratase (3HPCD) is an enzyme involved in the 3-HP/4-HB cycle by converting 3-hydroxypropionyl-CoA to acryloyl-CoA. To elucidate the molecular mechanism of 3HPCD from M. sedula (Ms3HPCD), we determined its crystal structure in complex with Coenzyme A (CoA). Ms3HPCD showed an overall structure and the CoA-binding mode similar to other enoyl-CoA hydratase (ECH) family enzymes. However, compared with the other ECHs, Ms3HPCD has a tightly formed α3 helix near the active site, and bulky aromatic residues are located at the enoyl-group binding site, resulting in the enzyme having an optimal substrate binding site for accepting short-chain 3-hydroxyacyl-CoA as a substrate. Moreover, based on the phylogenetic tree analysis, we propose that the 3HPCD homologues from the phylum Crenarchaeota have an enoyl-group binding pocket similar to that of bacterial short-chain ECHs.

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Citations

Apr 24, 2020·The Journal of Microbiology·Jisub HwangJun Hyuck Lee
May 6, 2021·Acta Crystallographica. Section F, Structural Biology Communications·Sivaraman PadavattanBagautdin Bagautdinov

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Methods Mentioned

BETA
X-ray
Size-exclusion chromatography
PCR
size exclusion chromatography
electrophoresis

Software Mentioned

PyMOL
Basic Local Alignment Search Tool ( BLAST )
AutoDock Vina
WinCoot
HKL2000
JLigand
BioNJ
MOLREP
PISA

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