Structural insights into a 20.8-kDa tegumental-allergen-like (TAL) protein from Clonorchis sinensis

Scientific Reports
Chang Hwa JoKwang Yeon Hwang

Abstract

Survival of Clonorchis sinensis, a cause of human clonorchiasis, requires tegument proteins, which are localized to the tegumental outer surface membrane. These proteins play an important role in a host response and parasite survival. Thus, these proteins are interesting molecular targets for vaccine and drug development. Here, we have determined two crystal structures of the calmodulin like domain (amino acid [aa] positions 1-81) and dynein light chain (DLC)-like domain (aa 83-177) of a 20.8-kDa tegumental-allergen-like protein from Clonorchis sinensis (CsTAL3). The calmodulin like domain has two Ca2+-binding sites (named CB1 and CB2), but Ca2+ binds to only one site, CB1. The DLC-like domain has a dimeric conformation; the interface is formed mainly by hydrogen bonds between the main chain atoms. In addition, we have determined full-length structure of CsTAL3 in solution and showed the conformational change of CsTAL3 induced by Ca2+ ion binding using small-angle X-ray scattering analysis and molecular dynamics simulations. The Ca2+-bound form has a more extended conformation than the Ca2+-free from does. These structural and biochemical analyses will advance the understanding of the biology of this liver fluke and may contrib...Continue Reading

References

Oct 12, 1979·Biochemical and Biophysical Research Communications·G A Jamieson, T C Vanaman
Apr 1, 1978·Pathology·H D Attwood, S T Chou
Jul 21, 1977·Zeitschrift Für Parasitenkunde·R Gönnert, P Andrews
Apr 1, 1995·Molecular and Biochemical Parasitology·M CamachoA Agnew
May 29, 1998·International Journal for Parasitology·P C Fan
Dec 16, 1998·Molecular and Biochemical Parasitology·M M MohamedA M Karim
Jul 30, 1999·Nature Structural Biology·J LiangJ Clardy
Dec 15, 2000·Current Opinion in Structural Biology·A Lewit-Bentley, S Réty
Apr 1, 1986·Kisaengch'unghak chapchi. The Korean journal of parasitology·Han Jong Rim
Dec 29, 2004·The Lancet Infectious Diseases·Zhao-Rong LunYue-Yi Fang
Feb 1, 2005·Parasite Immunology·C M FitzsimmonsDavid W Dunne
Sep 13, 2005·Journal of Helminthology·H-J Rim
Mar 21, 2006·International Journal for Parasitology·Jaap J Van HellemondAloysius G M Tielens
Nov 1, 2006·Current Opinion in Infectious Diseases·Alan Fenwick, Joanne P Webster
Jan 16, 2007·International Journal for Parasitology·Alex LoukasMark S Pearson
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Oct 16, 2008·Virchows Archiv : an International Journal of Pathology·Kee-Taek JangJae Hoon Lim
May 26, 2009·Acta Crystallographica. Section D, Biological Crystallography·Thomas C TerwilligerLi Wei Hung
Jul 14, 2009·General Physiology and Biophysics·Martin PelikanMichal Hammel
Jul 15, 2009·Clinical Microbiology Reviews·Jennifer Keiser, Jürg Utzinger
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Mar 6, 2010·Gastroentérologie Clinique Et Biologique·C de MartelS Franceschi
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
May 12, 2010·Nucleic Acids Research·Liisa Holm, Päivi Rosenström
Jul 12, 2011·Parasitology International·Sung-Tae Hong, Yueyi Fang
Mar 14, 2013·The Journal of Biological Chemistry·Pablo GallegoDavid Reverter
Jul 23, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Taís Nóbrega de SousaCristiana Ferreira Alves de Brito
Oct 31, 2013·Methods in Molecular Biology·Fabian Sievers, Desmond G Higgins
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Jun 11, 2014·Acta Crystallographica. Section F, Structural Biology Communications·M A F CostaR A P Nagem
Apr 1, 2012·Journal of Applied Crystallography·Maxim V PetoukhovDmitri I Svergun

❮ Previous
Next ❯

Citations

Mar 17, 2019·Cellular and Molecular Life Sciences : CMLS·Antonio VillaloboMartin W Berchtold

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
dot blots

Software Mentioned

GNOM
Pymol
SEDNTERP
PHASER
DALI
PHENIX
SEDFIT
ESPript
BILBOMD
PISA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.