Structural insights into Aspergillus fumigatus lectin specificity: AFL binding sites are functionally non-equivalent

Acta Crystallographica. Section D, Biological Crystallography
Josef HouserMichaela Wimmerová

Abstract

The Aspergillus fumigatus lectin AFL was recently described as a new member of the AAL lectin family. As a lectin from an opportunistic pathogen, it might play an important role in the interaction of the pathogen with the human host. A detailed study of structures of AFL complexed with several monosaccharides and oligosaccharides, including blood-group epitopes, was combined with affinity data from SPR and discussed in the context of previous findings. Its six binding sites are non-equivalent, and owing to minor differences in amino-acid composition they exhibit a marked difference in specific ligand recognition. AFL displays a high affinity in the micromolar range towards oligosaccharides which were detected in plants and also those bound on the human epithelia. All of these results indicate AFL to be a complex member of the lectin family and a challenging target for future medical research and, owing to its binding properties, a potentially useful tool in specific biotechnological applications.

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Citations

Nov 2, 2015·Applied Microbiology and Biotechnology·Jerica SabotičMarkus Künzler
Jun 23, 2020·Future Medicinal Chemistry·Harlei MartinTrinidad Velasco-Torrijos
Jun 21, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Son Thai LeMagdolna Csávás
Feb 9, 2021·European Biophysics Journal : EBJ·Josef HouserMichaela Wimmerova
Apr 23, 2021·Organic & Biomolecular Chemistry·Christophe DussouySébastien G Gouin

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Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.