Intron splicing is a fundamental biological process whereby noncoding sequences are removed from precursor RNAs. Recent work has provided new insights into the structural features and reaction mechanisms of two introns that catalyze their own splicing from precursor RNA: the group I and II introns. In addition, there is an increasing amount of structural information on the spliceosome, which is a ribonucleoprotein machine that catalyzes nuclear pre-mRNA splicing in eukaryotes. Here, we compare structures and catalytic mechanisms of self-splicing RNAs and we discuss the possible implications for spliceosomal reaction mechanisms.
Studies of point mutants define three essential paired nucleotides in the domain 5 substructure of a group II intron.
Photocrosslinking of 4-thio uracil-containing RNAs supports a side-by-side arrangement of domains 5 and 6 of a group II intron
Mutually exclusive distribution of IS1548 and GBSi1, an active group II intron identified in human isolates of group B streptococci
Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge
Effects of maturase binding and Mg2+ concentration on group II intron RNA folding investigated by UV cross-linking
Linking the group II intron catalytic domains: tertiary contacts and structural features of domain 3
RNA splicing: group I intron crystal structures reveal the basis of splice site selection and metal ion catalysis
Internal bulge and tetraloop of the catalytic domain 5 of a group II intron ribozyme are flexible: implications for catalysis
Self-splicing of a group IIC intron: 5' exon recognition and alternative 5' splicing events implicate the stem-loop motif of a transcriptional terminator
A three-dimensional model of a group II intron RNA and its interaction with the intron-encoded reverse transcriptase
RNase P without RNA: identification and functional reconstitution of the human mitochondrial tRNA processing enzyme
Maternally expressed gene 3 (MEG3) noncoding ribonucleic acid: isoform structure, expression, and functions
Prp8, the pivotal protein of the spliceosomal catalytic center, evolved from a retroelement-encoded reverse transcriptase
The structural stabilization of the κ three-way junction by Mg(II) represents the first step in the folding of a group II intron
Transition State Features in the Hepatitis Delta Virus Ribozyme Reaction Revealed by Atomic Perturbations
The brown algae Pl.LSU/2 group II intron-encoded protein has functional reverse transcriptase and maturase activities
Thermodynamics and kinetics of RNA tertiary structure formation in the junctionless hairpin ribozyme
Flexible Assembly of Engineered Tetrahymena Ribozymes Forming Polygonal RNA Nanostructures with Catalytic Ability.
Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.