Structural insights into specific crRNA G-rich sequence binding by Meiothermus ruber Cse2

Journal of Structural Biology
Su LiuY Adam Yuan

Abstract

CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats)-mediated defense against invading nucleic acids is a process recently discovered in prokaryotes, which includes recognition and incorporation of invading genetic elements, transcription and processing of CRISPR-RNA (crRNA) and targeting the invaders through base pair recognition. In the type I-E CRISPR-Cas system, Cse2 is proposed to provide a platform to facilitate the targeting of the invading dsDNA by crRNA. Here we report the crystal structure of Meiothermus ruber Cse2 at 2.8Å. M. ruber Cse2 adopts an α-helical bundle scaffold, harbors a positive surface for nucleic acid binding and a conserved dimer interface with strikingly low buried surface area. M. ruber Cse2 selectively binds to G-rich crRNA sequence, which is stripped off from the Cse2-crRNA and Cascade-crRNA complexes by ssDNA or dsDNA with complementary sequence. Stable M. ruber Cascade is readily formed by co-expression of M. ruber Cascade proteins together with G-rich crRNA in vitro. Docking of M. ruber Cse2 structures into the Escherichia coli Cascade Cryo-EM envelope reveals a curved elongated shallow groove for ssRNA binding, which adopts a similar dimer interface discovered by high-resolution...Continue Reading

References

May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Thean-Hock TangAlexander Hüttenhofer
Mar 29, 2005·Journal of Molecular Evolution·Francisco J M MojicaElena Soria
Sep 12, 2006·Journal of Structural Biology·Thomas D GoddardThomas E Ferrin
Mar 24, 2007·Science·Rodolphe BarrangouPhilippe Horvath
Aug 16, 2008·Science·Stan J J BrounsJohn van der Oost
Oct 31, 2008·RNA·Caryn HaleMichael P Terns
Dec 20, 2008·Science·Luciano A Marraffini, Erik J Sontheimer
Jan 15, 2009·Genes & Development·Jason CarteMichael P Terns
Oct 23, 2009·Bioinformatics·Christine RousseauJacques Nicolas
Dec 1, 2009·Cell·Caryn R HaleMichael P Terns
Jan 9, 2010·Science·Philippe Horvath, Rodolphe Barrangou
Feb 4, 2010·Nature Reviews. Genetics·Luciano A Marraffini, Erik J Sontheimer
Feb 5, 2010·Molecular Cell·Fedor V Karginov, Gregory J Hannon
Nov 5, 2010·Nature·Josiane E GarneauSylvain Moineau
Apr 2, 2011·Nature·Elitza DeltchevaEmmanuelle Charpentier
Apr 5, 2011·Nature Structural & Molecular Biology·Matthijs M JoreStan J J Brouns
May 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Blake WiedenheftJennifer A Doudna
May 10, 2011·Nature Reviews. Microbiology·Kira S MakarovaEugene V Koonin
May 17, 2011·Nature Structural & Molecular Biology·Dipali G SashitalJennifer A Doudna
May 17, 2011·Nature Structural & Molecular Biology·Emily M GesnerAndrew M Macmillan
Jan 10, 2012·Molecular Cell·Jing ZhangMalcolm F White
Apr 24, 2012·Molecular Cell·Dipali G SashitalJennifer A Doudna
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Jul 12, 2012·Nature Communications·Kirill A DatsenkoEkaterina Semenova
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Giedrius GasiunasVirginijus Siksnys
Nov 13, 2012·Nature Structural & Molecular Biology·Giuseppe NicastroAndres Ramos
Jan 22, 2013·The EMBO Journal·Tomas SinkunasVirginijus Siksnys
Oct 15, 2013·Molecular Cell·Christophe RouillonMalcolm F White
Aug 19, 2014·Nature Structural & Molecular Biology·Yanwu HuoAilong Ke

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