Structural insights into the acidophilic pH adaptation of a novel endo-1,4-β-xylanase from Scytalidium acidophilum

Biochimie
Catherine MichauxJ Wouters

Abstract

In this study, the crystal structure of a novel endo-1,4-β-xylanase from Scytalidium acidophilum, XYL1, was solved at 1.9Å resolution. This is one of the few solved crystal structures of acidophilic proteins. The enzyme has the overall fold typical to family 11 xylanases. Comparison of this structure with other homologous acidophilic, neutrophilic and alkalophilic xylanases provides additional insights into the general features involved in low pH adaptation (stability and activity). Several sequence and structure modifications appeared to be responsible for the acidophilic characteristic: (a) the presence of an aspartic acid H bonded to the acid/base catalyst (b) the nature of specifically conserved residues in the active site (c) the negative potential at the surface (d) the decreased number of salt bridges and H bonds in comparison with highly alkaline enzymes.

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Citations

Jan 19, 2016·Applied Microbiology and Biotechnology·Kusum Dhakar, Anita Pandey
Nov 10, 2011·Biotechnology Advances·Gabriel PaësJohnny Beaugrand
Nov 2, 2018·Bioscience, Biotechnology, and Biochemistry·Satoshi Wakai
Jul 1, 2020·Frontiers in Bioengineering and Biotechnology·Daochen ZhuJianzhong Sun
Apr 13, 2013·Biochemistry·Martin L LudwiczekLawrence P McIntosh

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