Structural insights into the regulation of SigB activity by RsbV and RsbW

IUCrJ
Deepak PathakDong Young Kim

Abstract

Bacillus subtilis SigB is an alternative sigma factor that initiates the transcription of stress-responsive genes. The anti-sigma factor RsbW tightly binds SigB to suppress its activity under normal growth conditions and releases it when nonphosphorylated RsbV binds to RsbW in response to stress signals. To understand the regulation of SigB activity by RsbV and RsbW based on structural features, crystal structures and a small-angle X-ray scattering (SAXS) envelope structure of the RsbV-RsbW complex were determined. The crystal structures showed that RsbV and RsbW form a heterotetramer in a similar manner to a SpoIIAA-SpoIIAB tetramer. Multi-angle light scattering and SAXS revealed that the RsbV-RsbW complex is an octamer in solution. Superimposition of the crystal structure on the SAXS envelope structure showed that the unique dimeric interface of RsbW mediates the formation of an RsbV-RsbW octamer and does not prevent RsbV and SigB from binding to RsbW. These results provide structural insights into the molecular assembly of the RsbV-RsbW complex and the regulation of SigB activity.

References

Jan 4, 1969·Nature·R R BurgessE K Bautz
Apr 1, 1994·Journal of Bacteriology·A Dufour, W G Haldenwang
Jan 1, 1993·Protein Engineering·G J Barton
Nov 18, 1998·FEMS Microbiology Reviews·M M Wösten
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·C A GrossB Young
Jun 16, 2001·Advances in Microbial Physiology·M Hecker, U Völker
Jun 27, 2002·The Journal of Biological Chemistry·Ruchira Chattopadhyay, Siddhartha Roy
Sep 25, 2002·Journal of Bacteriology·Olivier DelumeauMichael D Yudkin
Sep 3, 2003·Molecular Microbiology·Chien-Cheng ChenOlivier Delumeau
Dec 8, 2004·Microbiology·Shrin KuoW G Haldenwang
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Nov 24, 2007·Annual Review of Microbiology·Michael HeckerUwe Völker
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Sep 17, 2011·Journal of Chemical Information and Modeling·Roman A Laskowski, Mark B Swindells
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
Mar 13, 2013·Sub-cellular Biochemistry·Sarah E Barchinger, Sarah E Ades
Apr 29, 2014·The Journal of Physical Chemistry. B·Abhijit ChakrabortySaikat Chakrabarti
May 1, 2016·Nucleic Acids Research·Liisa Holm, Laura M Laakso
Jul 20, 2016·Nature Communications·Karthik V RajasekarColin Kleanthous
Apr 1, 2009·Journal of Applied Crystallography·Daniel Franke, Dmitri I Svergun
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Methods Mentioned

BETA
environmental stress
light
X-ray
size-exclusion chromatography
light-scattering
protein scattering

Software Mentioned

SUPCOMB
LIGPLOT
ALSCRIPT
phenix
PDBePISA
SWISS
refine
Phaser
DAMMIF
GNOM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.