Structural insights into the similar modes of Nrf2 transcription factor recognition by the cytoplasmic repressor Keap1.

Journal of Synchrotron Radiation
Balasundaram PadmanabhanShigeyuki Yokoyama

Abstract

The cytoplasmic repressor Keap1 regulates the function of transcription factor Nrf2 which plays critical roles in oxidative and xenobiotic stresses. The Neh2 domain of Nrf2 interacts with Keap1 at the bottom region of the Kelch/beta-propeller domain which is formed by double-glycine repeat and C-terminal region domains (Keap1-DC). The structure of Keap1-DC complexed with an Nrf2 peptide containing a conserved DLG motif has been determined at 1.9 A resolution. The Keap1-bound DLG peptide possesses a hairpin conformation, and it binds to the Keap1 protein at the bottom region of the beta-propeller domain. The intermolecular interaction occurs through their complementary electrostatic interactions. Comparison of the present structure with the recently reported Keap1-DC complex structure suggests that the DLG and ETGE motifs of Neh2 in Nrf2 bind to Keap1 in a similar manner but with different binding potencies.

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jul 30, 2002·The Journal of Biological Chemistry·Laurie M Zipper, R Timothy Mulcahy
Oct 1, 2003·Nature Genetics·Nobunao WakabayashiMasayuki Yamamoto
Apr 28, 2004·Free Radical Biology & Medicine·Ken ItohMasayuki Yamamoto
Nov 3, 2004·Trends in Molecular Medicine·Hozumi Motohashi, Masayuki Yamamoto
Dec 8, 2004·Archives of Biochemistry and Biophysics·Yasutake KatohMasayuki Yamamoto
Oct 7, 2005·Genome Biology·Peter J StogiosGilbert G Privé
Dec 16, 2005·Molecular and Cellular Biology·Akira KobayashiMasayuki Yamamoto
Mar 2, 2006·Molecular Cell·Balasundaram PadmanabhanMasayuki Yamamoto
Mar 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Balasundaram PadmanabhanShigeyuki Yokoyama
Aug 5, 2006·Advances in Enzyme Regulation·Makoto Kobayashi, Masayuki Yamamoto
Nov 4, 2006·Biological Chemistry·Kit I TongMasayuki Yamamoto

❮ Previous
Next ❯

Citations

Mar 3, 2011·Archives of Toxicology·Liam Baird, Albena T Dinkova-Kostova
Jul 12, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Young-Sam Keum, Bu Young Choi
Jan 22, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keiko TaguchiMasayuki Yamamoto
Mar 10, 2010·Journal of Surgical Oncology·Tsuyoshi TakahashiHiromi Wada
Nov 10, 2013·Free Radical Biology & Medicine·Hye-Kyung Na, Young-Joon Surh
Jul 11, 2013·Journal of Medicinal Chemistry·Anthony J WilsonChristopher J Moody
Dec 25, 2013·Molecular and Cellular Biology·Toshiaki FukutomiMasayuki Yamamoto
Dec 24, 2010·Cancer Research·Takafumi SuzukiMasayuki Yamamoto
Apr 3, 2020·Free Radical Biology & Medicine·Aleksandra KopaczAnna Grochot-Przeczek

❮ Previous
Next ❯

Datasets Mentioned

BETA
2DYH

Methods Mentioned

BETA
X-ray
ubiquitination
environmental stress

Software Mentioned

PROCHECK
Molrep
CCP4
HKL2000

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.