Structural Investigation into the Threading Intercalation of a Chiral Dinuclear Ruthenium(II) Polypyridyl Complex through a B-DNA Oligonucleotide.

Journal of the American Chemical Society
Simon D FairbanksMike P Williamson

Abstract

Herein we report the separation of the three stereoisomers of the DNA light-switch compound [{Ru(bpy)2}2(tpphz)]4+ (tpphz = tetrapyrido[3,2-a:2',3'-c:3″,2″-h:2‴,3‴-j]phenazine) by column chromatography and the characterization of each stereoisomer by X-ray crystallography. The interaction of these compounds with a DNA octanucleotide d(GCATATCG).d(CGATATGC) has been studied using NMR techniques. Selective deuteration of the bipyridyl rings was needed to provide sufficient spectral resolution to characterize structures. NMR-derived structures for these complexes show a threading intercalation binding mode with slow and chirality-dependent rates. This represents the first solution structure of an intercalated bis-ruthenium ligand. Intriguingly, we find that the binding site selectivity is dependent on the nature of the stereoisomer employed, with Λ RuII centers showing a better intercalation fit.

References

Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jan 7, 2000·Journal of Molecular Biology·M J PackerC A Hunter
Jul 18, 2001·Journal of the American Chemical Society·B OnfeltB Nordén
Oct 28, 2004·Journal of the American Chemical Society·Jeeyeon LeeBrent L Iverson
Feb 21, 2007·Angewandte Chemie·Pär NordellPer Lincoln
Nov 17, 2007·Nature Protocols·Axel T Brunger
Jan 9, 2008·Journal of the American Chemical Society·Daniel A LuttermanClaudia Turro
Jan 22, 2010·Organic & Biomolecular Chemistry·Philip WaywellMike P Williamson
Aug 4, 2010·Chemistry : a European Journal·Johanna AnderssonPer Lincoln
Oct 21, 2011·The Journal of Physical Chemistry. B·Johanna Andersson, Per Lincoln
Jul 24, 2013·Journal of the American Chemical Society·James P HallChristine J Cardin

❮ Previous
Next ❯

Citations

Jan 23, 2020·Chemical Communications : Chem Comm·Hiwa K SaeedJim A Thomas
Dec 9, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Arnab BhattacharyyaAkhil R Chakravarty
Dec 9, 2020·Angewandte Chemie·Jacobo Gómez-GonzálezM Eugenio Vázquez
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sandra RamotowskaMariusz Makowski
Jul 28, 2021·Chembiochem : a European Journal of Chemical Biology·Sanjoy Kumar SheetSnehadrinarayan Khatua
Aug 12, 2021·Angewandte Chemie·Matthew D NewtonDavid S Rueda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.