PMID: 9630666Jun 19, 1998Paper

Structural investigations of basic amphipathic model peptides in the presence of lipid vesicles studied by circular dichroism, fluorescence, monolayer and modeling

Biochimica Et Biophysica Acta
C MangavelJ A Reynaud

Abstract

A cationic amphiphilic peptide made of 10 leucine and 10 lysine residues, and four of its fluorescent derivatives in which leucines were substituted by Trp residues at different locations on the primary sequence have been synthesized. The interactions of these five peptides with neutral anionic or cationic vesicles were investigated using circular dichroism, steady state and time-resolved fluorescence with a combination of Trp quenching by brominated lipid probes, monolayers, modeling with minimization and simulated annealing procedures. We show that all the five peptides interact with neutral and anionic DMPC, DMPG, DOPC or egg yolk PC vesicles. The binding takes place whatever the peptide conformation in solution is. In the case of DMPC bilayers the binding free energy DeltaG is estimated at -8 kcal mole-1 and the number of phospholipid molecules involved is about 20-25 per peptide molecule. Peptides are bound as single-stranded alpha helices orientated parallel to the bilayer surface. In the anchoring of phospholipid head groups around the peptides, the lipid molecules are not smeared out in a plane parallel to the membrane surface but are organized around the hydrophilic face of the alpha helices like 'wheat grains around a...Continue Reading

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Citations

Feb 15, 2001·The Biochemical Journal·J J CheethamA J Czernik
Feb 15, 2007·PloS One·Antonin LamazièreJesus Ayala-Sanmartin
Apr 10, 2008·PloS One·Antonin LamazièreJesus Ayala-Sanmartin
Apr 10, 2012·Analytical Biochemistry·Pavlína Novotná, Marie Urbanová
Aug 30, 2005·Bioelectrochemistry·Alina FrantescuEberhard Neumann
Oct 9, 2004·Biochimica Et Biophysica Acta·Daniel PerssonBengt Nordén
May 17, 2005·Biochimica Et Biophysica Acta·Sónia Troeira Henriques, Miguel A R B Castanho
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Oct 20, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·André Hädicke, Alfred Blume
Jan 1, 2004·Chinese Science Bulletin = Kexue Tongbao·Yuhua YuanQimin Chen

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