PMID: 9445057Jan 28, 1998Paper

Structural localization of the E3 glycoprotein in attenuated Sindbis virus mutants

Journal of Virology
Angel ParedesW Chiu

Abstract

We have determined the three-dimensional structures of the wild-type Sindbis virus and two of its mutants that retain the E3 sequence within PE2. Using difference imaging between these mutants and the wild-type virus, we have assigned a location for the 64-amino-acid sequence corresponding to E3 in the mutant spike complex. In the wild-type virus, the spike is composed of an E1-E2 heterotrimer. The E3 protein was found to protrude midway between the center of the spike complex and the tips. Based on these results and the work of others, we propose a distribution for the functional domains of the spike proteins within the structure of wild-type Sindbis virus. Within the structure of the virus, the E1 domains form the central portion of the spike complex, while the tips are formed by the E2 domains that flare out from the center of the complex. The structural similarity between these Sindbis virus mutants and Ross River virus suggests that E3 may also be present in the latter, which is also a member of the Alphavirus genus.

References

Dec 1, 1991·Journal of Virology·S Ubol, D E Griffin
Mar 1, 1991·Journal of Virology·R P Anthony, D T Brown
Aug 1, 1991·Journal of Virology·P Levy-Mintz, M Kielian
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T S BakerM Bina
May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·I de Curtis, K Simons
May 27, 1971·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R A Crowther
Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·J H StraussJ E Darnell
Feb 1, 1984·Virology·E G StraussJ H Strauss
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C M Rice, J H Strauss
Sep 1, 1980·The Journal of General Virology·A ZiemieckiK Simons
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·T J SmithT S Baker
Feb 18, 1994·Journal of Molecular Biology·C Vénien-Bryan, S D Fuller
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A M ParedesB V Prasad
Mar 1, 1993·Virology·R J Schoepp, R E Johnston
May 1, 1996·Virology·M Mulvey, D T Brown
Jan 1, 1996·Journal of Structural Biology·P A Thuman-Commike, W Chiu
Jan 1, 1996·Journal of Structural Biology·J A Lawton, B V Venkataram Prasad

❮ Previous
Next ❯

Citations

Oct 22, 2002·Journal of Virology·Wei ZhangMichael G Rossmann
Apr 6, 2005·The Journal of Cell Biology·Elena ZaitsevaLeonid V Chernomordik
Sep 3, 2009·Future Microbiology·Joyce JoseRichard J Kuhn
Dec 3, 2010·Nature·Long LiMichael G Rossmann
Mar 13, 2012·Annual Review of Biophysics·David Veesler, John E Johnson
Oct 14, 2014·Advances in Virology·Raquel HernandezAngel Paredes
Oct 29, 2017·Archives of Virology·Haritha Ramya KrishnavajhalaHans Heidner
Dec 6, 2017·Proceedings of the National Academy of Sciences of the United States of America·Moh Lan YapMichael G Rossmann
Jun 1, 2005·Virology·William B KlimstraHans W Heidner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Virology
Megan M ParrottSuchetana Mukhopadhyay
Proceedings of the National Academy of Sciences of the United States of America
T J SmithT S Baker
Journal of Virology
Anthony J BattistiM G Rossmann
© 2021 Meta ULC. All rights reserved