Structural Mimicry Drives HIV-1 Rev-Mediated HERV-K Expression.

Journal of Molecular Biology
Ina P O'CarrollYun-Xing Wang

Abstract

Expression of the Human Endogenous Retrovirus Type K (HERV-K), the youngest and most active HERV, has been associated with various cancers and neurodegenerative diseases. As in all retroviruses, a fraction of HERV-K transcripts is exported from the nucleus in unspliced or incompletely spliced forms to serve as templates for translation of viral proteins. In a fraction of HERV-K loci (Type 2 proviruses), nuclear export of the unspliced HERV-K mRNA appears to be mediated by a cis-acting signal on the mRNA, the RcRE, and the protein Rec-these are analogous to the RRE-Rev system in HIV-1. Interestingly, the HIV-1 Rev protein is able to mediate the nuclear export of the HERV-K RcRE, contributing to elevated HERV-K expression in HIV-infected patients. We aimed to understand the structural basis for HIV Rev-HERV-K RcRE recognition. We examined the conformation of the RcRE RNA in solution using small-angle X-ray scattering (SAXS) and atomic force microscopy (AFM). We found that the 433-nt long RcRE can assume folded or extended conformations as observed by AFM. SAXS analysis of a truncated RcRE variant revealed an "A"-shaped topological structure similar to the one previously reported for the HIV-1 RRE. The effect of the overall topolo...Continue Reading

References

Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M L ZappM R Green
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H P BogerdW C Greene
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·R LöwerR Kurth
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J YangB R Cullen
May 29, 2000·Molecular and Cellular Biology·B R Cullen
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Mar 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Robert BelshawMichael Tristem
Nov 12, 2005·Journal of Virology·Jennifer A MertzJaquelin P Dudley
Oct 28, 2006·AIDS Research and Human Retroviruses·Rafael Contreras-GalindoYasuhiro Yamamura
Jan 30, 2007·PLoS Pathogens·Young Nam Lee, Paul D Bieniasz
Feb 1, 2007·AIDS Research and Human Retroviruses·Rafael Contreras-GalindoYasuhiro Yamamura
Oct 7, 2008·Nucleic Acids Research·Matthias MüllnerStanislav Indik
Apr 29, 2009·Bioinformatics·Kévin DartyYann Ponty
Aug 8, 2009·Nature·Joseph M WattsKevin M Weeks
Aug 15, 2009·The EMBO Journal·Dagmar MohrDirk Görlich
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Michael A DiMattiaJonathan M Grimes
Oct 19, 2010·Nature Structural & Molecular Biology·Matthew D DaughertyAlan D Frankel
Sep 29, 2011·Molecular BioSystems·Pau Bernadó, Dmitri I Svergun
Oct 28, 2011·Journal of Virology·Rafael Contreras-GalindoDavid M Markovitz
Jan 18, 2012·Retrovirology·Antoinette C van der Kuyl
Feb 23, 2012·Cold Spring Harbor Perspectives in Medicine·Jonathan Karn, C Martin Stoltzfus
May 18, 2012·Journal of Virology·Marta J Gonzalez-HernandezDavid M Markovitz
Nov 13, 2012·The Journal of Clinical Investigation·R Brad JonesMario A Ostrowski
Apr 27, 2013·Nature·Robert P Rambo, John A Tainer
May 4, 2013·Nucleic Acids Research·Sabrina LusvarghiStuart F J Le Grice
Nov 19, 2013·Cell·Xianyang FangYun-Xing Wang

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

HIV/AIDS-Related Malignancies

HIV/AIDS infection increases the risk of non-communicable diseases common in the aged including HIV/AIDS-related malignancies. Discover the latest research in HIV/AIDS-related malignancies.