Structural modifications of the prostate in hypoxia, oxidative stress, and chronic ischemia

Korean journal of urology
Portia ThurmondKazem Azadzoi

Abstract

Clinical studies have reported a correlation between pelvic ischemia and voiding dysfunction in elderly men. The aim of this study was to identify and compare prostate structural modifications in cultured cells and in a rabbit model after exposure to hypoxia, oxidative stress, and chronic ischemia. Cultured human prostate smooth muscle cells (SMCs), epithelial cells (ECs), and stromal cells (SCs) were incubated under normoxia, hypoxia, and oxidative stress conditions by use of a computerized oxycycler system. We developed a rabbit model of chronic prostate ischemia by creating aorto-iliac arterial atherosclerosis. Markers of oxidative stress were examined by using fluorometric analysis and enzyme immunoassay. Prostate structure was examined by using Masson's trichrome staining and transmission electron microscopy (TEM). Lipid peroxidation was found in SMCs exposed to hypoxia and in all cell types exposed to oxidative stress. We identified protein oxidation in ECs exposed to hypoxia and in all cell types exposed to oxidative stress. Markers indicating oxidative damage were present in chronically ischemic rabbit prostate tissue. These reactions were associated with DNA damage. Prostate ischemia resulted in epithelial atrophy, los...Continue Reading

Citations

Sep 30, 2016·The Prostate·Yasuhito FunahashiMomokazu Gotoh
Nov 19, 2019·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Shogo ShimizuMotoaki Saito
May 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcello LocatelliGiustino Orlando
Nov 18, 2019·Pathology, Research and Practice·Won Jin Cho, Jung-Soo Pyo

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Methods Mentioned

BETA
transmission electron microscopy
ELISA
MDA

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