Structural organization of a gene encoding a novel calmodulin-binding kinesin-like protein from Arabidopsis

Gene
A S ReddyI S Day

Abstract

Kinesin-like calmodulin-binding protein (KCBP) is a recently identified microtubule motor protein that appears to be unique to plants. Here we report isolation and sequence analysis of a gene encoding Arabidopsis KCBP. KCBP gene contains 21 exons and 20 introns. All exons except exon 3 are short (94-272 nt). Exons 1-9 code for the globular tail region whereas the coiled-coil region is coded by exons 10-15. The conserved motor domain is coded by exons 16-20. Calmodulin-binding domain that is present in the C-terminal region of the protein and unique to KCBP is coded by the last exon. The size of introns ranged from 71 (intron 17) to 320 (intron 19) nucleotides. As in most plant introns, the content of AT is very high in all introns (up to 76%). Phylogenetic analysis of KCBP using motor domain sequence grouped KCBP with other known C-terminal microtubule motor proteins. However, Arabidopsis KCBP together with its homologs from potato and tobacco constitute a distinct group within the C-terminal subfamily of motors which is consistent with structural and functional features of KCBP.

References

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Citations

Aug 26, 1998·Cell Motility and the Cytoskeleton·B E DeavoursR A Walker
Dec 23, 1999·Current Opinion in Plant Biology·B KostN H Chua
Jun 3, 2004·Nature Reviews. Molecular Cell Biology·Martin Hülskamp
Mar 15, 2006·Trends in Plant Science·Marleen VanstraelenDanny Geelen
Jun 30, 2005·Animal Health Research Reviews·Armando E Hoet, Linda J Saif
Apr 25, 2012·Annals of the New York Academy of Sciences·Ajit Varki, Pascal Gagneux

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