Structural organization of DNA in chlorella viruses

PloS One
Timo WulfmeyerTobias Meckel

Abstract

Chlorella viruses have icosahedral capsids with an internal membrane enclosing their large dsDNA genomes and associated proteins. Their genomes are packaged in the particles with a predicted DNA density of ca. 0.2 bp nm(-3). Occasionally infection of an algal cell by an individual particle fails and the viral DNA is dynamically ejected from the capsid. This shows that the release of the DNA generates a force, which can aid in the transfer of the genome into the host in a successful infection. Imaging of ejected viral DNA indicates that it is intimately associated with proteins in a periodic fashion. The bulk of the protein particles detected by atomic force microscopy have a size of ∼60 kDa and two proteins (A278L and A282L) of about this size are among 6 basic putative DNA binding proteins found in a proteomic analysis of DNA binding proteins packaged in the virion. A combination of fluorescence images of ejected DNA and a bioinformatics analysis of the DNA reveal periodic patterns in the viral DNA. The periodic distribution of GC rich regions in the genome provides potential binding sites for basic proteins. This DNA/protein aggregation could be responsible for the periodic concentration of fluorescently labeled DNA observed ...Continue Reading

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Jan 21, 2016·Food and Environmental Virology·Dayanand B Sajjan, Shivayogeppa B Hinchigeri
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Datasets Mentioned

BETA
JF411744.1

Methods Mentioned

BETA
Fluorescence
light microscopy
AFM
force measurements
atomic force microscopy

Software Mentioned

Image Processing Software
NumPy for
Mascot Server PMF
BioPyhton
Masslynx
Mascot Server
Image Metrology A / S

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