Structural organization of the dynein-dynactin complex bound to microtubules

Nature Structural & Molecular Biology
Saikat ChowdhuryGabriel C Lander

Abstract

Cytoplasmic dynein associates with dynactin to drive cargo movement on microtubules, but the structure of the dynein-dynactin complex is unknown. Using electron microscopy, we determined the organization of native bovine dynein, dynactin and the dynein-dynactin-microtubule quaternary complex. In the microtubule-bound complex, the dynein motor domains are positioned for processive unidirectional movement, and the cargo-binding domains of both dynein and dynactin are accessible.

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Methods Mentioned

BETA
ion-exchange chromatography

Software Mentioned

EMAN
Appion
UCSF Chimera
Relion
SPARX
EMAN2
CTFFindv3
MSA
ISAC
diffmap

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