Structural organization of the TRP1 gene of Phycomyces blakesleeanus: implications for evolutionary gene fusion in fungi

Gene
H T ChoiM Jayaram

Abstract

The complete nucleotide (nt) sequence of the cloned TRP1 gene from Phycomyces blakesleeanus is reported. The gene encodes a trifunctional polypeptide that represents a sequential fusion of glutamine amidotransferase (TrpG), indoleglycerolphosphate synthetase (TrpC), and phosphoribosylanthranilate isomerase (TrpF) activities from the amino- to the carboxy terminus. This genetic organization is characteristic of filamentous fungi in general. The transcription start sites and the polyadenylation sites of the gene have been mapped. The two predominant TRP1 transcripts have a short leader of 13 and 18 nt, while minor species with significantly longer leaders are also detectable. Approximately 50 bp and 70 bp upstream from the major transcription start points, sequences that match the canonical eukaryotic TATA and CAAT boxes, respectively, are found. Two major polyadenylation signals are located approximately 50 and 70 nt downstream from the translational stop. Three closely clustered minor polyadenylation sites map to roughly 120-150 bp 3' to the termination codon. The TRP1 gene is the first and only Phycomyces gene that has been cloned and sequenced. The information regarding the promoter and terminator of a Phycomyces gene derived...Continue Reading

References

Dec 11, 1979·Biochemistry·S C ElginL E Hood
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B Vogelstein, D Gillespie
Jun 1, 1975·Bacteriological Reviews·I P Crawford
Sep 16, 1976·Nature·N J Proudfoot, G G Brownlee
Sep 23, 1976·Molecular & General Genetics : MGG·A P EslavaK Kong
Oct 18, 1976·Molecular & General Genetics : MGG·F J Murillo, E Cerdá-Olmedo
Feb 10, 1975·Biochimica Et Biophysica Acta·R L Dusenbery
Apr 1, 1988·Molecular & General Genetics : MGG·T Suárez, A P Eslava
Jan 1, 1985·Molecular & General Genetics : MGG·E J MullaneyW E Timberlake
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J L Revuelta, M Jayaram
Jan 1, 1986·Annual Review of Microbiology·R HütterJ A DeMoss
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Jan 1, 1973·Molecular & General Genetics : MGG·K BergmanE Cerdá-Olmedo
Jun 4, 1974·Biochemistry·V GlisinC Byus
Dec 1, 1967·Journal of Bacteriology·R Hütter, J A DeMoss
Mar 1, 1969·Bacteriological Reviews·K BergmanW Shropshire
Jan 11, 1980·Nucleic Acids Research·C BenoistP Chambon
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M G Kidwell
Oct 1, 1981·European Journal of Biochemistry·F J MurilloE Cerda-Olmedo
Jan 1, 1994·Progress in Lipid Research·E Cerdá-Olmedo, J Avalos
Jan 1, 1969·Plant Physiology·R I Gamow, W Goodell
May 1, 1979·Genetics·M Delbrück, T Ootaki
Jul 1, 1983·Current Genetics·I López-Díaz, E D Lipson

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Citations

Jul 22, 2005·Applied Microbiology and Biotechnology·Tamás PappEnrique A Iturriaga
Sep 26, 1996·Gene·J AvalosS Brenner
Sep 12, 2000·FEMS Microbiology Letters·R NakaiH Shibai
Jan 29, 2000·European Journal of Biochemistry·E NavarroS Torres-Martínez
Aug 2, 1994·Molecular & General Genetics : MGG·V GarreS Torres-Martínez
Oct 1, 1989·Applied and Environmental Microbiology·J S HorngJ J Pestka

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