Structural origin of the drastic modification of second harmonic generation intensity pattern occurring in tail muscles of climax stages xenopus tadpoles

Journal of Structural Biology
Gaëlle RecherFrançois Tiaho

Abstract

Second harmonic generation (SHG) microscopy is a powerful tool for studying submicron architecture of muscles tissues. Using this technique, we show that the canonical single frequency sarcomeric SHG intensity pattern (SHG-IP) of premetamorphic xenopus tadpole tail muscles is converted to double frequency (2f) sarcomeric SHG-IP in metamorphic climax stages due to massive physiological muscle proteolysis. This conversion was found to rise from 7% in premetamorphic muscles to about 97% in fragmented muscular apoptotic bodies. Moreover a 66% conversion was also found in non-fragmented metamorphic tail muscles. Also, a strong correlation between predominant 2f sarcomeric SHG-IPs and myofibrillar misalignment is established with electron microscopy. Experimental and theoretical results demonstrate the higher sensitivity and the supra resolution power of SHG microscopy over TPEF to reveal 3D myofibrillar misalignment. From this study, we suggest that 2f sarcomeric SHG-IP could be used as signature of triad defect and disruption of excitation-contraction coupling. As the mechanism of muscle proteolysis is similar to that found in mdx mouse muscles, we further suggest that xenopus tadpole tail resorption at climax stages could be used ...Continue Reading

References

Jan 1, 1992·Acta Neuropathologica·M J CullenJ B Harris
Aug 1, 1965·The Journal of Cell Biology·S G Page
Jan 1, 1997·British Medical Bulletin·N A Thornberry
May 8, 1999·Free Radical Biology & Medicine·A KashiwagiK Utsumi
Jan 16, 2002·The Journal of Physiology·Bradley S Launikonis, D George Stephenson
Apr 17, 2002·The Journal of Physiology·Ella W YeungDavid G Allen
Mar 25, 1957·The Journal of Biophysical and Biochemical Cytology·K R PORTER, G E PALADE
Dec 21, 2004·Redox Report : Communications in Free Radical Research·Masayasu InoueKozo Utsumi
Mar 15, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Isaline RoweLaurent Coen
Nov 1, 2005·Biophysical Journal·Sergey V PlotnikovWilliam A Mohler
Mar 6, 2007·Development, Growth & Differentiation·Kenta FujimotoYoshio Yaoita
Mar 31, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jaishri Menon, Roy Rozman
Nov 22, 2008·Journal of Biomedical Optics·Nicole PrentVirginijus Barzda
Nov 22, 2008·Journal of Biomedical Optics·Sergey V PlotnikovWilliam A Mohler
Nov 17, 2009·Journal of Applied Physiology·Bert BlaauwCarlo Reggiani
Apr 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·V NucciottiF S Pavone
Feb 23, 2011·Development, Growth & Differentiation·Atsuko Ishizuya-Oka
Feb 23, 2011·Biomedical Optics Express·Gaëlle RecherFrançois Tiaho
Sep 13, 2011·IEEE Transactions on Bio-medical Engineering·Christoph S GarbeOliver Friedrich
Jun 13, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keisuke NakajimaYoshio Yaoita
Nov 8, 2012·The Journal of Pathology·Andreas ButtgereitOliver Friedrich
Apr 5, 2013·The FEBS Journal·Terence A Partridge
May 21, 2013·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jennifer JohnsonJaishri Menon
Jul 3, 2013·Journal of Cell Science·Isuru D Jayasinghe, Bradley S Launikonis
May 24, 2014·Journal of Biomedical Optics·Patricia Garcia-CanadillaEduard Gratacos

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Jul 25, 2012·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·Wei-Liang ChenChen-Yuan Dong
May 14, 2021·Scientific Reports·Dimitrios KapsokalyvasMarc A M J van Zandvoort

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