Structural phenotyping of stem cell-derived cardiomyocytes

Stem Cell Reports
Francesco Silvio PasqualiniKevin Kit Parker

Abstract

Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during striated muscle cell development. To test our metrics, we analyzed the localization of the contractile protein α-actinin in a variety of primary and stem-cell derived cardiomyocytes. Further, we combined these metrics with data mining algorithms to unbiasedly score the phenotypic maturity of human-induced pluripotent stem cell-derived cardiomyocytes.

References

Feb 1, 1970·The American Journal of Cardiology·N J Sissman
Sep 8, 2007·Science·Adam W FeinbergKevin Kit Parker
Jul 19, 2008·Circulation Research·Kevin Kit ParkerLeslie Tung
Feb 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thouis R JonesDavid M Sabatini
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matthew D TreiserPrabhas V Moghe
Jun 26, 2010·Circulation Research·Sheng WeiLong-Sheng Song
Dec 3, 2010·PLoS Computational Biology·Lior ShamirIlya G Goldberg
Mar 11, 2011·PLoS Computational Biology·Anna GrosbergKevin Kit Parker
Apr 21, 2012·Science Translational Medicine·Ning SunJoseph C Wu
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jun 30, 2012·Nature Methods·Kevin W EliceiriAnne E Carpenter
Jul 10, 2012·Biomechanics and Modeling in Mechanobiology·Sean P SheehyKevin Kit Parker
Mar 7, 2013·Stem Cells and Development·Scott D LundyMichael A Laflamme
May 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Megan L McCainKevin Kit Parker
Sep 14, 2013·Circulation Research·Li Qian, Deepak Srivastava
Mar 29, 2014·Stem Cell Reports·Sean P SheehyKevin Kit Parker
Apr 1, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Megan L McCainKevin Kit Parker
Apr 16, 2014·Biomaterials·Megan L McCainKevin Kit Parker
Aug 16, 2014·Cell·Patrick CahanJames J Collins

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Citations

Feb 10, 2016·The Journal of Cell Biology·Yvonne Aratyn-SchausKevin Kit Parker
Dec 18, 2015·Advanced Healthcare Materials·Raghavendra PalankarMihaela Delcea
Dec 15, 2015·Advanced Drug Delivery Reviews·A K CapulliK K Parker
Oct 5, 2016·The Journal of Cell Biology·Alexander P NesmithKevin Kit Parker
Jan 26, 2016·PloS One·Renita E HortonKevin Kit Parker
Mar 29, 2018·PloS One·Francesco Silvio PasqualiniKevin Kit Parker
Jan 18, 2018·Biofabrication·Janna C NawrothKevin Kit Parker
Sep 1, 2017·Journal of Biomechanical Engineering·Hongyan YuanKevin Kit Parker
Oct 28, 2016·Journal of Biomechanical Engineering·Nancy K DrewAnna Grosberg
Dec 13, 2018·ELife·Aidan M FenixDylan T Burnette
Jan 21, 2018·Scientific Reports·Matthew D SutcliffeJeffrey J Saucerman
Apr 12, 2019·Circulation Research·Jason W MiklasDeok-Ho Kim
Aug 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Sarah R ClippingerMichael J Greenberg
Jul 9, 2016·Science·Sung-Jin ParkKevin Kit Parker
Oct 8, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ricardo H PiresOliver Otto
Mar 3, 2020·Integrative Biology : Quantitative Biosciences From Nano to Macro·Andrew P PetersenMegan L McCain
Mar 5, 2020·PLoS Computational Biology·Tessa Altair MorrisAnna Grosberg
May 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ville J KujalaKevin Kit Parker
Apr 25, 2019·Nature Biomedical Engineering·Luke A MacQueenKevin Kit Parker
Jan 4, 2017·BioMed Research International·Francesco S PasqualiniKevin Kit Parker
May 11, 2018·Analytical and Bioanalytical Chemistry·Seungkuk AhnKevin Kit Parker
Mar 28, 2017·Experimental Biology and Medicine·Sean P SheehyKevin K Parker
Jul 13, 2019·Journal of Biological Engineering·Anh H NguyenHung Cao
Sep 15, 2020·PLoS Genetics·Aviva LevitasIoannis Karakikes
Apr 10, 2021·Science·Masahito OgawaKazu Kikuchi
Apr 26, 2019·ACS Applied Materials & Interfaces·Brisa PeñaLuisa Mestroni
Jun 10, 2019·ACS Biomaterials Science & Engineering·Feng ZhangNing-Ping Huang

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Methods Mentioned

BETA
Extraction
feature extraction

Software Mentioned

ImageJ
Matlab
AUTOCAD
FIJI

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