Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions

Science
Georg K A HochbergJustin L P Benesch

Abstract

Oligomeric proteins assemble with exceptional selectivity, even in the presence of closely related proteins, to perform their cellular roles. We show that most proteins related by gene duplication of an oligomeric ancestor have evolved to avoid hetero-oligomerization and that this correlates with their acquisition of distinct functions. We report how coassembly is avoided by two oligomeric small heat-shock protein paralogs. A hierarchy of assembly, involving intermediates that are populated only fleetingly at equilibrium, ensures selective oligomerization. Conformational flexibility at noninterfacial regions in the monomers prevents coassembly, allowing interfaces to remain largely conserved. Homomeric oligomers must overcome the entropic benefit of coassembly and, accordingly, homomeric paralogs comprise fewer subunits than homomers that have no paralogs.

References

Jun 1, 1992·Computer Applications in the Biosciences : CABIOS·D T JonesJ M Thornton
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 17, 1968·Nature·M Kimura
Aug 26, 1998·Nature·K K KimS H Kim
May 20, 1999·Molecular Biology and Evolution·E R Waters, E Vierling
Nov 10, 2001·Nature Structural Biology·R L van MontfortE Vierling
Oct 17, 2003·Nature·Sina GhaemmaghamiJonathan S Weissman
Jul 23, 2004·The Journal of Chemical Physics·Hans W HornTeresa Head-Gordon
Jan 14, 2005·Bioinformatics·Federico AbascalDavid Posada
Jan 11, 2007·The Journal of Chemical Physics·Giovanni BussiMichele Parrinello
Apr 7, 2007·Genome Biology·Jose B Pereira-LealSarah A Teichmann
May 8, 2007·Journal of Molecular Biology·Nobuhiko TokurikiDan S Tawfik
Feb 16, 2008·Science·William E BalchJeffery W Kelly
Jun 20, 2008·Nature·Emmanuel D LevySarah A Teichmann
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Feb 11, 2010·The Journal of Biological Chemistry·Eman BashaElizabeth Vierling
Apr 22, 2010·Proteins·Kresten Lindorff-LarsenDavid E Shaw
May 5, 2010·Protein Science : a Publication of the Protein Society·Arthur LaganowskyDavid Eisenberg
Oct 23, 2010·Molecular Cell·Klaus RichterJohannes Buchner
Aug 16, 2011·Journal of Molecular Biology·Andrew J BaldwinJustin L P Benesch
Jan 11, 2012·Nature·Gregory C FinniganJoseph W Thornton
May 12, 2012·Topics in Current Chemistry·Gillian R HiltonJustin L P Benesch
Mar 27, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gillian R HiltonJustin L P Benesch
May 15, 2013·Nucleic Acids Research·Hamish McWilliamRodrigo Lopez
Jun 12, 2013·Nucleic Acids Research·Daniel W A BuchanDavid T Jones
Jul 3, 2013·Structure·Matteo T Degiacomi, Matteo Dal Peraro
Jul 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Michael Lynch
Feb 25, 2014·Nature Biotechnology·Seesandra V RajagopalaPeter Uetz
Sep 12, 2014·Molecular & Cellular Proteomics : MCP·Georgia Orfanoudaki, Anastassios Economou
Apr 1, 2012·Journal of Applied Crystallography·Maxim V PetoukhovDmitri I Svergun
Dec 11, 2014·Annual Review of Biochemistry·Joseph A Marsh, Sarah A Teichmann
Dec 17, 2014·Methods in Molecular Biology·Frances D L KondratJustin L P Benesch
Jan 24, 2015·Science·Mathias UhlénFredrik Pontén

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Citations

Oct 24, 2018·The Journal of Biological Chemistry·Indu SanthanagopalanElizabeth Vierling
Nov 6, 2018·The Journal of Biological Chemistry·Martin HaslbeckJohannes Buchner
Feb 14, 2019·Cell Stress & Chaperones·Serena CarraRobert M Tanguay
Nov 27, 2019·The Journal of Biological Chemistry·Evgeny V MymrikovJohannes Buchner
Apr 17, 2020·The New Phytologist·Elizabeth R Waters, Elizabeth Vierling
Apr 18, 2020·Cell Stress & Chaperones·Igor Obuchowski, Krzysztof Liberek
Aug 28, 2020·PLoS Computational Biology·Saurav Mallik, Dan S Tawfik
May 16, 2019·Annual Review of Microbiology·Axel MogkBernd Bukau
Apr 8, 2020·Cell Stress & Chaperones·Miranda P Collier, Justin L P Benesch
Jul 17, 2020·International Journal of Molecular Sciences·Natalia A ChebotarevaBoris I Kurganov
Nov 27, 2018·Biochemistry. Biokhimii︠a︡·L K MuranovaN B Gusev
Sep 27, 2018·Cold Spring Harbor Perspectives in Medicine·Jonathan B RothbardLawrence Steinman
Feb 2, 2021·The Journal of Biological Chemistry·Evgeny V MymrikovJohannes Buchner
Dec 11, 2020·Nature·Georg K A HochbergJoseph W Thornton
May 23, 2021·Nature Communications·Chuanyang YuWilson Chun Yu Lau
May 23, 2021·Journal of Molecular Biology·Artur PirógKrzysztof Liberek
Jun 25, 2021·Chemical Science·Anna SinelnikovaCarl Caleman

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