Structural processes during starch granule hydration by synchrotron radiation microdiffraction

Biomacromolecules
H LemkeChristian Riekel

Abstract

Starch granule hydration has been examined on the level of a single potato starch granule by static and dynamic synchrotron radiation (SR) microdiffraction techniques. A cryofrozen, hydrated granule was mapped through a 5 microm SR-beam in order to investigate its internal organization. The edge of the granule showed fiber texture scattering due to radially oriented amylopectin helices. The variation of fiber texture across the granule center supports the model of concentric shells. The crystalline phase appears, however, to increase strongly toward the granule center due to a random amylopectin fraction, which could be related to crystallization of short-range ordered amylopectin during hydration. During gelatinization, the shell structure breaks down and remaining fiber-textured amylopectin domains belong probably to the swollen starch granule envelope. Hydration of a granule was initiated by a microdrop generator and followed in situ by SR-microdiffraction. A fast hydration process with a half time of about 7 s seems to reflect the porous nature of starch granules. The size of the hydrated domains suggests that this process is limited to the level of amylopectin side chain clusters. Longer hydration times are assumed to invo...Continue Reading

References

Aug 1, 1975·Biopolymers·K H Gardner, J Blackwell
May 20, 1988·Journal of Molecular Biology·A ImbertyV Tran
Oct 18, 2000·International Journal of Biological Macromolecules·Y SaitoJ L Putaux
Apr 13, 2006·Journal of Synchrotron Radiation·T Y Teng, K Moffat

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Citations

Dec 3, 2008·The Review of Scientific Instruments·R GraceffaC Riekel
Jan 24, 2006·Journal of Structural Biology·Henri ChanzyChristian Riekel
Jun 28, 2014·Journal of Synchrotron Radiation·Angelo AccardoChristian Riekel
Sep 1, 2016·Carbohydrate Polymers·Leonardo GalvisTapani Vuorinen
Jun 28, 2007·Journal of Experimental Botany·Lester W YoungMartin J T Reaney
Nov 20, 2020·Scientific Reports·Francesco SpinozziSerge Perez
Feb 8, 2021·International Journal of Biological Macromolecules·Jing WangQinlu Lin
Mar 15, 2006·Biomacromolecules·Andreas BlennowMaria Labanowska

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