Structural properties of a multifunctional T-shaped RNA domain that mediate efficient tomato bushy stunt virus RNA replication

Journal of Virology
Debashish RayK Andrew White

Abstract

In positive-strand RNA viruses, 5' untranslated regions (5' UTRs) mediate many essential viral processes, including genome replication. Previously, we proposed that the 5'-terminal portion of the genomic leader sequence of Tomato bushy stunt virus (TBSV) forms an RNA structure containing a 3-helix junction, termed the T-shaped domain (TSD). In the present study, we have carried out structure-function analysis of the proposed TSD and have confirmed an important role for this domain in mediating efficient viral RNA amplification. Using a model TBSV defective interfering RNA replicon and a protoplast system, we demonstrated that various TSD subelements contribute to the efficiency of viral RNA replication. In particular, the stabilities of all three stems (S1, S2, and S4) forming the 3-helix junction are important, while stem-loop 3-a terminal extension of S2-is largely dispensable. Additionally, some of the sequences forming the 3-helix junction are required in an identity-dependent manner. Thus, both secondary structure and nucleotide identity are important for TSD-mediated viral RNA replication. Importantly, these results are fully consistent with the dual functions we defined previously for the sequences corresponding to loops...Continue Reading

References

Jan 1, 1994·Advances in Virus Research·M RussoG P Martelli
Jun 1, 1997·The Journal of General Virology·L Rubino, M Russo
Dec 13, 2002·Journal of Virology·Debashish Ray, K Andrew White
Oct 9, 2003·The EMBO Journal·Judit PoganyPeter D Nagy

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Citations

Jul 17, 2007·Nature Reviews. Microbiology·Ian BrierleyRobert J C Gilbert
Oct 21, 2011·Journal of Virology·Kunj B PathakPeter D Nagy
Feb 28, 2006·Journal of Virology·Chi-Ping ChengPeter D Nagy
May 18, 2006·Annual Review of Phytopathology·W Allen Miller, K Andrew White
May 30, 2013·PLoS Pathogens·Baodong WuK Andrew White
Sep 1, 2005·Molecular Plant Pathology·Yoshimi Yamamura, Herman B Scholthof
Mar 13, 2014·Virology·Pui Kei K Lee, K Andrew White
Feb 1, 2005·Virology·Olena A Chernysheva, K Andrew White
Apr 21, 2011·RNA Biology·Zhenghe Li, Peter D Nagy
Aug 5, 2021·Journal of Virology·Chaminda D GunawardeneK Andrew White

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